摘要
以鱼鳞为对象,采用胃蛋白酶在酸性(乙酸、盐酸、柠檬酸)条件下进行酶解得到胶原蛋白进行性质研究。探讨酸浓度、酶解时间、酶解温度及料液比对提取率的影响,并对酶解得到的胶原蛋白冷冻干燥提纯进行性质研究。试验结果表明:提取率高低依次为乙酸>柠檬酸>盐酸,其中乙酸提取各因素影响效应为乙酸浓度>提取温度>料液比>提取时间。最佳试验方案为乙酸浓度0.6mol/L,提取温度36℃,提取时间6h,料液比1:30,此时羟脯氨酸含量为2.7522mg/mL,提取率73.32%。性质研究结果表明:胶原蛋白的溶解性很好,乳化性则是盐酸提取最高59.6%,乳化稳定性都较好,而吸油性、起泡性则为乙酸提取最佳,分别为3mL/g,90%。
This experiment with fish processing scales for research object of scraps, Adopt pepsin respectively in acid （hydrochloric acid, acetic acid, citric acid） conditions zymolysing get collagen. Explore acid concentration, enzymatic hydrolysis time, enzymatic hydrolysis temperature and the influence of material liquid extraction ratio, And to get zymolysing collagen freeze-drying purification for further study of nature. Test results show that： Extraction rate from high to low in turn is citric acid〉acetic acid 〉hydrochloric acid, The factors which effect of acetic acid extract order size is acetic acid concentration〉extraction temperature〉 material liquid〉extraction time. The scheme for best test is acetic acid concentration 0.6 mol/L, Extraction temperature is 36 %, Extraction time is 6 h, Than liquid materials is 1：30, Now hydroxyproline content is 2.7522 mg/mL, Extraction rate reached 73.32%. Nature research results indicate： Collagen solubility is very good, Emulsification is hydrochloric acid extraction is highest, 59.6%, Emulsion stability are good, and suck oily, foaming ability is the best acetic acid extraction, Achieved respectively is 3 mL/g, 90%.
出处
《食品科技》
CAS
北大核心
2012年第11期141-148,共8页
Food Science and Technology
基金
西昌学院研究生项目(XY09-2A02)
关键词
鱼鳞
酸
提取
性质
fish scales
acid
extraction
properties
