摘要
选择 3组随机引物 ,利用随机扩增多态性DNA技术 ,对江苏省 1988～ 1997年分离到的 72株分属于M 1、D2、E1和D1噬菌体型的伤寒菌株进行分子流行病学研究。综合 3组引物扩增的结果分析 ,2 6株M 1型噬菌体型的伤寒菌呈 7个基因型 ,其中 1988～ 1991年分离到的 14株菌表现为 7个基因型 ,M 1型菌株在长期的进化过程中 ,产生了许多基因型 ,造成了80年代末和 90年代初江苏省大范围的伤寒高强度流行 ,1992年后M 1型菌株的基因型趋于同一化 ,伤寒疫情也逐步呈下降趋势 ;D2型菌株基因型保持较强的稳定性 ;E1型和D1型伤寒菌随时间的变迁 ,其基因型也随着改变。
Random amplified polymorphic DNA(RAPD)bacterial fingerrinting method was used for the genotype taxon of 72 strains of salmonella typhi(S.typhi)of phaye types M1,D2,E1 and D1 from Jiangsu Province among 1988～1997.The results showed that the isolates of phage type M1 were divided into seven genotypes.The 14 isolates from 1988 to 1991 were classified into seven genotypes,it suggests that the DNA polymorphism of isolates attributed to the epidemic of typhi in Jiangsu Province in the end of 80's and the begin of 90's.After 1992,the 91 67%(11/12)of the isolates were in the same group,the incidence of typhi were decreased in the same time.The genotype of D2 isolates were stable.The genetic pattens of E1 and D1 changed in the different years.Ths use of RAPD analysis enable us to recognise differences in DNA patterns among isolates of S.typhi of similar phage types and provide the epidemiological information more accurate and detailed.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2000年第5期465-467,共3页
Chinese Journal of Public Health
基金
江苏省科学技术委员会资助!课题
BS960 4 0
