摘要
目的:建立同时测定地呋乳膏中呋喃西林和醋酸地塞米松含量的方法。方法:采用高效液相色谱法。色谱柱为AgilentTC-C18柱,流动相为甲醇-水(60:40),流速为0.8mL.min-1,检测波长为240nm,柱温为30℃,进样量为10μL。结果:呋喃西林和醋酸地塞米松检测浓度分别在10.11～202.20μg.mL-(1r=0.9999)和2.51～50.20μg.mL-(1r=0.9999)范围内与峰面积积分值呈良好的线性关系;呋喃西林平均加样回收率为100.03%(RSD=1.06%,n=9),醋酸地塞米松平均加样回收率为99.55%(RSD=0.96%,n=9)。结论:该方法快速、简便,重复性好,结果可靠,可用于地呋乳膏的质量控制。
OBJECTIVE：To determine the method for the content determination of nitrofurazone and dexamethasone acetate in Difu cream.METHODS：HPLC was applied.Agilent TC-C18 column was used with mobile phase consisted of methanol-water（60：40） at the flow rate of 0.8 mL·min-1.The detection wavelength was set 240 nm,and the column temperature was 30 ℃.The injection volume was 10 μL.RESULTS：The linear range of nitrofurazone was 10.11～202.20 μg·mL-1（r=0.999 9） and that of dexamethasone acetate was 2.51～50.20 μg·mL-1（r=0.999 9）.The average recovery of nitrofurazone was 100.03%（RSD=1.06%,n=9）,and that of dexamethasone acetate was 99.55%（RSD=0.96%,n=9）,respectively.CONCLUSION：The method is proved to be rapid,simple,reproducible and reliable.It is suitable for the quality control of Difu cream.
出处
《中国药房》
CAS
CSCD
2012年第44期4208-4210,共3页
China Pharmacy
