摘要
研究大孔树脂纯化胡桃楸种仁壳黄酮工艺条件,并对纯化前后黄酮的抗氧化活性进行了测定。以黄酮回收率为指标,考察大孔树脂对胡桃楸种仁壳黄酮的静态吸附与解吸、动态吸附与解吸性能;采用分光光度法,测定胡桃楸种仁壳黄酮对ABTS.+、DPPH.、.OH的清除能力和对金属离子的鳌合作用。结果表明,HPD-600大孔树脂适合纯化胡桃楸种仁壳黄酮,纯化工艺为:上样量1/3BV、吸附时间2h、上样pH6,上样浓度1.2mg/mL,用4BV的蒸馏水和2BV的90%乙醇洗脱,纯化后黄酮纯度由18.6%提高到37.7%。胡桃楸种仁壳黄酮具有明显的清除ABTS+.的能力,有较强清除DPPH.和.OH的能力,对铁离子具有一定的鳌合作用,且抗氧化活性与黄酮含量呈剂量效应关系。经与VC、Na2EDTA和BHT比较,抗氧化活性强于BHT,弱于VC、Na2EDTA。胡桃楸种仁壳黄酮具有显著的抗氧化作用。
The purified condition of flavonoids from seed shell of Juglans mandshurica was investigated by macroporous resin,and antioxidant activity of flavonoids were evaluated.Absorption and desorption of macroporous resin on flavonoids were investigated as recovery yield of total flavonoids as index.Additionally,scavenging ability of flavonoids on ABTS＋·,DPPH·,·OH and chelating effect on ferrous ions were determined by spectrophotometry to evaluate antioxidant activity.Results showed that using HPD-600 resin to purify flavonoids from seed shell of Juglans mandshurica,the optimal conditions were as follows：quantity of liquid sample 1/3BV,time of absorption 2h,pH6,concentration of liquid sample 1.2mg /mL,bed of macroporous resin were eluted using 4BV distilled water and 2BV 90% ethanol,purity of flavonoids was increased from 18.6% to 37.7%.Flavonoids from seed shell of Juglans mandshurica could significantly scavenge ABTS＋·,could scavenge DPPH· and ·OH,and had chelating effect on ferrous ion,there were dose-effect relationship.Compared with VC,Na2EDTA and BHT,antioxidant activity was stronger than BHT,weaker than VC and Na2EDTA.Flavonoids from seed shell of Juglans mandshurica had significantly antioxidant effect.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第22期113-118,122,共7页
Science and Technology of Food Industry
基金
中央高校基本科研业务费专项(LD09C14)
关键词
胡桃楸种仁壳
黄酮
纯化
抗氧化
Juglans mandshurica
seed shell
flavonoids
purification
antioxidant
