摘要
目的探讨褪黑素对新生大鼠缺氧缺血性脑损伤(HIBD)的保护作用。方法 Wistar大鼠48只,5日龄时结扎左侧颈总动脉,吸入氧氮混合气体50min制作成HIBD模型,随机分成生理盐水组,褪黑素组,缺血缺氧(HI)+生理盐水组,HI+褪黑素组。褪黑素腹腔注射共3次,每次5 mg/kg,第1次在结扎动脉前,第2次在吸入氧氮混合气体前给予,第3次在HIBD模型制作24h后给予。大鼠在HIBD模型制作后72h被处死,取脑作免疫组化染色,判断脑灰质(microtubule-associatedprotein-2,MAP-2)、脑白质(myelin basic protein,MBP)损伤;HIBD模型制作后7周作Y迷宫记忆功能测试。结果褪黑素能明显减轻HIBD大鼠脑灰质MAP-2和脑白质MBP损伤,还可提高大鼠的长期记忆能力和运动协调能力。结论褪黑素对HIBD大鼠大脑损伤有明显的短期和长期保护作用。
Objective To investigate neuroprotective effect of melatonin on newborn rat brain after hypoxia-ischemia brain damage （HIBD）. Methods Forty-eight 5-day-old Wistar rats were randomly divided into physiological saline group, melatonin group, hypoxia-ischemia （HI） ＋ saline group and HI ＋ melatonin group. The HIBD rat models were induced by unilateral ligation of the left common carotid artery followed by 50 rain inhalation of mixed oxygen and nitrogen. Melatonin was intraperitoneally injected three times with a dose of 5mg/kg before artery ligation, before ischemia and after 24h HI immediately. The rats were sacrified after 7211 HI and the immunohistochemical staining was applied to brain tissue. Micrombule-associated protein-2 （MAP-2） and myelin basic protein （MBP） were evaluated as indicators for damages in grey matter and white matter, respectively. The memory abilities of the rats were measured through Y maze test at 7 weeks after HI. Results Melatonin treatment reduced the injury of grey matter and white matter significantly, and improved the long-term memory ability and motor coordination. Conclu- sions Melatonin has a strong short and long-term protection effects on HI damaged brain.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2012年第11期1067-1070,共4页
Journal of Clinical Pediatrics
