摘要
目的研究刺五加叶不同部位在不同浓度下的体外抗氧化活性。方法采用清除ABTS+自由基、DPPH自由基评价法和测定还原性法,利用紫外分光光度计法测定刺五加叶的总提液、10%、30%和50%大孔吸附树脂醇洗脱部位在不同浓度下的抗氧化能力。结果刺五加叶不同部位抗氧化活性的强弱顺序为:30%部位>10%部位>总提液>50%部位,随着各部位浓度的增加,抗氧化能力增强。ABTS+自由基和DPPH自由基模型中,在浓度为0.64mg/mL时,30%的部位对ABTS+DPPH自由基模最大清除率分别达到99.541%、95.331%,与同浓度VC的清除率接近,其次浓度0.64mg/mL的10%部位的最大清除率分别达到96.324%、88.033%。在还原性法测定中30%部位在浓度为0.64mg/mL时,吸光度为2.269,与VC较接近,同浓度时10%部位的吸光度为2.155,次于30%部位。结论刺五加叶抗氧化能力较好,各种评定方法结合分析,其30%部位最好,可以开发为抗氧化剂,为进一步研究和开发刺五加叶提供了科学依据。
Objective Study on different Fractions of Acanthopanax Senticosus（Rupr.etMaxim.） Harms Leaves at different concentrations,and determine its antioxidative activities.Methods The antioxidative activities of Acanthopanax Senticosus compared with VC were investigated employing various methods established in vitro systems such as DPPH（1,1-diphenyl-2-picrylhydrazyl） radical ABTS（2,2-azo-bis-（3-ethylbenzothiozoline-6-sulphonic acid） radical scavenging assay and reducing power assay measured by ultraviolet spectro-photometer.Results The elution fractions of Acanthopanax Senticosus exhibited significant antioxidant properties on ABTS free radical,DPPH radical scavenging systems,and good reduction.The inhibition of freeing radical scavenging ability of the different elution fractions from strong to weak is： 30%fraction10%fraction the total extracts50%fraction.The antioxidant properties of different fractions depended well on the their dose.With the increasing of the concentration various fractions,the antioxidative capacity was enhanced.The inhibition of ABTS radical scavenging was 99.541% at the concentration of 0.64mg/mL in 30% fraction,and the inhibition of DPPH radical scavenging was 95.331% at the same concentration in 30% fraction,both close to VC.The inhibition in 10% fraction was weaker than 30% fraction,it was 96.324%、88.033% respectively.In the reducing power assay,the absorbance was about closed to VC that was 2.269 of 30% fraction,and the 10% fraction was 2.155.Conclusion Different fractions of Acanthopanax Senticosus showed significant antioxidant properties,the 30% ethanol fraction exhibited highest antioxidant activities in different investigation systems.The present studies offered a scientific record that 30% ethanol fraction would be developped to be an antioxidant.
出处
《人参研究》
2012年第3期11-15,共5页
Ginseng Research
基金
国家科技部资助攻关项目(2005BA741C)
