摘要
忍冬木层孔菌是木层孔菌属的一种药用真菌,目前对它的研究少有报道。以液体培养为基础,研究忍冬木层孔菌培养过程中生物量、胞内外多酚含量的变化,以及胞内外提取物抗氧化活性的变化。结果显示,菌丝体生物量在培养168–216h时达到最大值(8.33g/L);胞内多酚和胞外多酚含量分别在216h和168h时达到最大值(15.60mg GAE/g和106.76mg GAE/L);液体培养胞内外提取物的抗氧化活性与多酚含量正相关,多酚含量高时提取物对DPPH[1,1-Diphenyl-2-picrylhydrazyl radical2,2-Diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl]自由基的抑制率也高。
Phellinus lonicericola is a medicinal fungus belonging to the Hymenochaetaceae. Few reports on the fungus were published previously. The changes of biomass and polyphenolic content in mycelia (intracellular polyphenol IP) and culture broth (extracellular polyphenol EP), and the varieties of antioxidant activities of mycelial extracts and culture broth were studied during the liquid culture of P lonicericola. The results showed that the highest levels for myeelial biomass of P lonicericola reached 8.33g/L at 168 and 216h, for IP 15.60mg GAE/g at 216h and EP 106.76mg GAE/L at 168h. The extracts of the mycelia showed antioxidant activity against DPPH radical with capacity positively related to the polyphenolic content.
出处
《菌物学报》
CAS
CSCD
北大核心
2012年第6期933-939,共7页
Mycosystema
基金
教育部新世纪优秀人才支持计划资助
关键词
药用菌
生物量
还原糖含量
DPPH自由基清除活性
medicinal fimgi, biomass, content of reducing sugar, activity of DPPH radical scavenging