摘要
采用营养液培养方法,研究外源NO供体硝普钠(SNP)处理对50μmol·L-1Cu2+胁迫下番茄幼苗Cu的亚细胞分布和化学形态的影响.结果表明:Cu胁迫下,番茄幼苗的生物量和株高显著降低33.7%和23.1%,外源NO能够显著缓解这种抑制作用,但各器官中Cu的含量和累积量仍显著升高.Cu胁迫下,番茄幼苗各器官的Cu含量和累积量大小依次为根系>叶片>茎>叶柄,幼苗根系吸收的Cu向地上部的转运大幅降低,外源NO只能缓解而不能消除这种作用.外源NO可以使Cu胁迫下幼苗各器官的液泡和细胞壁中Cu含量显著上升,细胞器中Cu含量降低,从而减轻过多Cu对胞质生理生化代谢的伤害,增强组织细胞对Cu的耐性.外源NO可以提高番茄幼苗根系中醋酸提取态铜(FHAc)、茎中氯化钠提取态铜(FNaCl)、叶柄中FHAc、叶片中乙醇提取态铜(FE)和FNaCl的含量,降低水溶态铜(FW)的含量与比例,以降低过多铜的生物毒性.
A nutrient solution culture experiment was conducted to NO donor （sodium nitroprusside） on the subeellular distribution and in tomato seedlings under the stress of 50 μmol . L-1 of Cu2＋（ CuC12 mass and plant height of tomato seedlings decreased by 33.7% and nous NO alleviated this inhibition effect significantly, but the Cu con the seedling study the effects of exogenous chemical form of copper （Cu） ）. Under this stress, the bio- 23.1%, respectively. Exoge- centration and accumulation in organs still had a significant increase. Under the Cu stress, the Cu concentration and accumulation in the seedling organs were in the order of root 〉 leaf 〉 stem 〉 petiole. Exogenous NO limited the absorbed Cu transferred from root to shoot, but could not remove this translocation. Ex- ogenous NO increased the Cu concentration in vacuole and cell wall significantly, and decreased the concentration in organelle, which lessened the damage of Cu on the regular metabolic balance in cytoplasm and increased the tolerance of organelle against Cu. Exogenous NO increased the acetic acid-extractable Cu （F_HAc） in root, sodium chloride-extractable Cu （F_NaCl） in stem, F_HAc in peti- ole, and ethanol-extractable Cu （FE ） and F_NaCl in leaf, while decreased the concentration and dis- tribution of water-extractable Cu （Fw） in different organs, which efficiently reduced the bio-toxicity of excessive copper.
出处
《应用生态学报》
CAS
CSCD
北大核心
2012年第11期3033-3039,共7页
Chinese Journal of Applied Ecology
基金
国家自然科学基金项目(31201619)
现代农业产业技术体系专项资金项目(CARS-25-D)
泰安市科技发展计划项目(32606)资助
关键词
番茄幼苗
NO
CU胁迫
亚细胞分布
化学形态
tomato seedling
NO
copper stress
subcellular distribution
chemical form.
