摘要
目的观察远志提取物远志总皂苷(TEN)对D-半乳糖致衰鹅膏蕈氨酸(IBO)诱导阿尔茨海默病(AD)模型大鼠突触可塑性的影响,并探讨其可能机制。方法将雄性Wistar大鼠随机分为对照组、AD模型组及TEN高、低剂量组,每组8只。通过电生理实验测定各组大鼠长时程增强(LTP)的变化,用免疫组织化学方法测定海马CA1区神经元N-甲基-D-天冬氨酸受体2A亚基(NR2A)积分吸光度(IOD)值,间接观察其表达情况,并进行各组间比较。结果高频刺激(HFS)后1 min、30 min和60 min对照组LTP值为(203.17±7.468)%、(178.15±8.110)%和(164.17±7.026)%,AD模型组LTP值〔(168.63±10.809)%、(120.12±7.382)%和(102.88±2.357)%〕较对照组显著降低(均P<0.05)。TEN低剂量组LTP值为(177.67±14.038)%、(141.83±4.956)%和(121.17±4.792)%,TEN高剂量组LTP值为(192.00±2.449)%、(168.00±2.449)%和(141.75±9.251)%,均较AD模型组升高(P<0.05)。与对照组(30.12±3.45)比较,AD模型组IOD值(11.74±1.69)显著降低(P<0.05);与AD模型组比较,TEN组低剂量组、高剂量组IOD值(分别为20.78±2.66、25.86±2.98)均明显增高(P<0.05),且TEN高剂量组高于TEN低剂量组(P<0.05)。结论 TEN可能使海马CA1区LTP升高和NR2A表达增加,从而改善AD大鼠突触可塑性。
Objective To observe the effects of tenuigenin （TEN） in polygala on synaptic plasticity in Alzheimer disease （AD） rats induced by ibotenic acid and aged by D-gal and to investigate the mechanism underlying the effects of TEN in polygala on synaptic plasticity. Methods Thirty-two male Wistar rats were divided randomly into four groups, the control group, the model group, the high dose TEN group and the low dose TEN group. Magnitude of long-term potentiation （LTP） was determined by comparing the average field exciatatory postsynaptic potential （fEPSP） amplitude over the baseline period with that after high-frequency stimulation （HFS） induction in the CA1 region of hippocampus. Then the immunohischemical changes of N- methyl-D-aspartate receptor 2A subunit （NR2A） in CA1 region of hippocampus were examined. Results After the HFS for 1 minute, 30 minutes and 60 minutes, LTP in the control group respectively reached （203.17± 7.47） %, （178. 15±8.11）% and （164.17± 7.03）%, while LTP of the model group only respectively reached （168.63±10.809）%, （120.12 ± 7. 382） % and （102.88±2.357）%, was significantly lower than the control group （P（0.05）. I.TP in high dose TEN in polygalae group respectively reached （192.00±2. 449）%, （168.00 ±2.449）% and （141.75±9. 251）%, which were higher （P〈0.05） than the low dose group＇ s （177.67±14. 038）%, （141.83±4. 956）% and （121.17±4. 792）%. Compared with the model group, both of the high and low dose group＇ s LTP were obviously improved （P〈0.05）. The integral optical density values of the control group, the AD model group, the high dose group and the low dose group were respectively 30.12±3.45, 11.74 ±1.69, 25.86!2.98 and 20. 78±2.66. Compared with the control group （30.12±3.45）, the expression of NR2A in the AD model group （11.74±1.69） was obivously less （P〈0.05）. Compared with the model group,the expression of NR2A was significantly more in both the high dose group （25.86 ± 2.98） and the low dose group （20.78±2.66） （P〈0.05, respectively）. Furthermore, the expression of NR2A was significantly more in the high dose group than the low dose group （P〈0.05）. Conclusions TEN in polygala may significantly enhance the LTP and increase the expression of NR2A in AD rats, and improve the synaptic plasticity in AID rats.
出处
《中国神经免疫学和神经病学杂志》
CAS
北大核心
2012年第6期449-452,共4页
Chinese Journal of Neuroimmunology and Neurology
基金
国家自然基金面上项目(81173455)
