摘要
以参薯的带节茎段为外植体、MS为基本培养基,研究了参薯的组织培养快速繁殖技术。结果表明,不含任何激素的MS培养基能诱导外植体的腋芽萌发,萌芽率达100%;萌发的茎段在MS+AD80mg·L-1的培养基上增殖效果较好,50d可增殖4倍;继代培养得到的带节茎段在MS+6-BA1.0mg·L-1+NAA0.1mg·L-1+AD80mg·L-1的培养基上能诱导出不定芽,将其芽点转接到MS+80mg·L-1AD培养基上,不定芽能抽生,且芽体健壮;不定芽在MS+NAA0.1mg·L-1的培养基上能形成完整植株,生根率达100%;V土:V沙:V草木灰=2:2:1的混合基质移栽参薯组培苗的效果较佳,移栽存活率可达88.3%。
Dioscorea alata L. were mass propagated through tissue culture on different MS media with the nodal stem segments as explants. The explants were induced to form axillary buds at an initiation rate of 100% when cultured on the MS medium without any auxins. The initiated nodal shoots were rapidly multiplied in the subcul- ture basic medium supplemented with 80 mg · L-1 AD （adenine sulfate） and 4 times of nodal shoots could be produced at day 50 of subculture. Adventitious buds were induced from the nodal segments subcultured in the basic medium supplemented with 6 - BA 1.0 mg · L-1, NAA0.1 mg · L-1 and 80 mg · L-1 AD, and produced robust shoots when cultured on the medium supplemented with 80 mg · L-1 AD. The adventitious shoots were rooted on the MS medium containing NAA0.1 mg · L-1 to form complete plants, and the rooting rate was 100%. The rooted plantlets were transplanted onto a mixed substrate of garden soil, sand and plant ash（ V： V： V=2：2：1 ） and resulted in 88.3% survival.
出处
《热带生物学报》
2012年第3期271-275,共5页
Journal of Tropical Biology
基金
高等学校博士学科点专项科研基金(20114601110001)
农业部热带作物种质资源保护项目(11RZ
ZY-26)
关键词
参薯
组织培养
带节茎段
不定芽
移栽
Dioscorea alata
nodal stem
adventitious bud
transplanting
