摘要
目的观察百草枯(PQ)中毒大鼠肺组织中4-羟基壬烯醛(4-HNE)的表达。方法将48只SD大鼠按随机数字表法分为A、B两组,每组24只。B组PQ灌胃(80mg/kg)染毒建立SD大鼠肺损伤模型,A组等量生理盐水灌胃。灌胃后12、24、48、72h观察两组大鼠肺组织病理改变及检测肺组织中4-HNE表达。结果肺组织的病理变化:A组无明显变化;B组肺泡毛细血管扩张,伴弥漫性肺出血,肺泡腔塌陷,肺泡腔内炎性细胞浸润。4-HNE在肺组织中的表达:B组明显高于A组(P<0.01)。结论 PQ中毒时4-HNE表达增强,4-HNE可能为PQ中毒大鼠的肺损伤主要标志之一。
Objective To investigate the 4-hydroxynonenal（4-HNE） expression changes in lungs of paraquat（PQ）-intoxicated rates. Methods Forty-eight healthy Sprague-Dawley rats were randomly divided into two groups., group A and group B,with 24 rats in each group. The model of lung injury was established by intragastric PQ（80 mg/kg） administration in group B, and same quantity of normal saline was administered intragastrically in group A. Pathological changes in lung tissue were observed in 12,24, 48 and 72 hours after treatment. The pathological changes and the expression of 4-HNE in lung tissue were detected. Results There was no significant change in A group. The pathological changes,including alveolar capillary expansion, diffuse alveolar hem orrhage and alveolar inflammation cell infiltration, were found in lungs of rats in group B. Compared with group A, the expression of 4-HNE in lung tissue significantly increased in group B（P〈0. 01）. Conclusion The expression of 4-HNE increased in PQ intoxica- ted rats. 4-HNE may play an important role in ratsr lung injury induced by PQ.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第32期3394-3395,3414,F0002,共4页
Chongqing medicine
基金
江西省卫生厅科技项课题(20111015)
