摘要
【目的】明确稻曲菌粗毒素的主要活性组分;比较不同地区稻曲球的稻曲毒素A含量;研究不同稻曲菌菌株产毒素A量与致病力的相关性。【方法】分别用丙酮、甲醇、水等不同极性溶剂从稻曲球中萃取制备粗毒素,并用小麦胚根抑制法检测其活性;高效液相色谱(HPLC)检测采集自全国224份稻曲球样品的毒素A含量,紫外扫描及液相色谱-质谱(LC-MS)验证稻曲毒素A;用人工接种的方法,测定52个稻曲菌株对感病品种两优培九的致病力;应用SPSS统计软件对稻曲菌产毒素A量与致病力进行相关性分析。【结果】用水和甲醇萃取得到的粗毒素活性较高,丙酮最低;质谱测定组分Ⅱ的分子量为673.9,结合紫外吸收光谱初步证明粗毒素的主要活性成分是稻曲毒素A。采集自不同地区的224份稻曲球样品的毒素A含量有明显差异。不同稻曲菌株的产毒素A量与致病力相关性不显著(相关系数R=0.222,显著性概率P=0.114)。【结论】稻曲球的活性成分中含有稻曲毒素A;不同地区稻曲球中的毒素A含量有显著差异;稻曲菌菌株的产毒素A量与其致病力没有显著相关性。
[ Objective ] The objective of this study is to define the active components of crude toxin produced by Ustilaginoidea virens, to analyze the ustiloxin A production of false smut balls in different areas, and to discover the correlation between pathogenicity and ustiloxin A production of the fungus. [Method] The crude toxin of U. virens was extracted from false smut balls by aeetune, methanol and water, respectively, and the toxic effect was assayed and indicated by inhibition to wheat seed germination. The active components of the crude toxins were studied by liquid chromatography mass-spectrometry （LC-MS） and UV absorption spectra. Ustiloxin A production of 224 false smut balls collected from different areas in China was analyzed by high performance liquid chromatography （HPLC）. The pathogenicity of 52 isolates of U. virens was examined through inoculating the susceptible rice cultivars, Liangyoupeijiu. The correlation between pathogenicity and ustiloxin A production of the fungus was analyzed by using SPSS software. [Result] The crude toxin extracted with water and methanol had higher inhibition to wheat radicle growth with the inhibition rate of 59.6% and 52.9%, respectively, while the crude toxin extracted with acetone had lower inhibition. The molecular weight of the active ingredient Ⅱ was 673.9 by determination with LC-MS. Combined with the UV spectra assay, ingredient Ⅱ could be proved to be ustiloxin A. It was found that there was a significant difference in the ustiloxin A production among false smut balls collected from different areas. However, correlation analysis indicated that no significant correlation between pathogenicity of the fungus and its ustiloxin A production （R=0.222, P=0.114 ） . [Conclusion] Water extract of false smut balls caused by U. virens contained ustiloxin A. And a significant difference in ustiloxin A production was found in false smut balls collected from different areas. There may be no association between pathogenicity and ustiloxin A production of the fungus.
出处
《中国农业科学》
CAS
CSCD
北大核心
2012年第22期4720-4727,共8页
Scientia Agricultura Sinica
基金
国家公益性行业(农业)科研专项(200903039-2)
江苏自主创新特色产业技术体系(CX(12)1003)
关键词
稻曲菌
粗毒素
稻曲毒素A
致病力
Ustilaginoidea virens
crude toxin
ustiloxin A
pathogenicity
