摘要
目的:观察苦参碱对豚鼠心室肌动作电位及钠通道的作用,探讨其抗心律失常的作用机制。方法:采用标准微电极记录技术记录动作电位,采用膜片钳全细胞记录技术记录通道电流。结果:苦参碱(10μmol/L;50μmol/L;100μmol/L)浓度依赖性延长APD50和APD90,降低APA(n=8);乌头碱明显延长APD50和APD90,苦参碱(100μmol/L)能逆转乌头碱对动作电位的过度延长作用(n=7);苦参碱(50μmol/L)抑制IN a,与对照组相比,其IN a电流密度在-45mV时从(-65.14±4.2)pA/pF降低到(-46.39±4.8)pA/pF(n=10)。结论:苦参碱能逆转乌头碱对动作电位时程的延长作用并能抑制IN a。
Objective：To observe the effect of Matrine on action potential and sodium channel in guinea pig papillary muscles thus to clarify its mechanisms of antiarrythmic effects.Methods：Conventional microelectrode technique was applied to record the action potential and the whole-cell patch clamp recording technique was used to record the change of sodium channel current.Results Matrine prolonged the action potential duration at 50%,90% repolazation（APD50,APD90） in a dose-dependent manner（P0.01,n=8） and decreased APA（P0.05,n=8）;Aconitine significantly prolonged the action potential.Matrine（100μmol/L） reversed action potential duration induced by the aconitine（n=7）.Matrine（50μmol/L） decreased the sodium channelcurrent density from（-65.14±4.2）pA/pF to（-46.39±4.8） pA/pF（-45mV）（n=10）.Conclusion Matrine reversed the changes of action potential duration induced by aconitine and decreased sodium channel current.
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2012年第11期1219-1221,共3页
JOURNAL OF BASIC CHINESE MEDICINE
基金
广西自然科学基金资助项目(桂科自0991154)
关键词
苦参碱
乌头碱
动作电位
心律失常
钠通道
matrine
aconitine
action potential
arrhythmic
sodium channel
