摘要
提出了一种基于微流控芯片的生物样品循环给样方法,利用聚二甲基硅氧烷(PDMS)软光刻工艺制作了包括环形蠕动微泵和电磁微阀的微流控芯片,通过与免疫探针芯片的集成,实现了对探针芯片的循环给样及在其上的免疫样品富集。采用不同浓度的羊抗兔IgG(FITC标记)样品与兔IgG探针免疫结合3 min,得到循环流动比静态吸附的荧光光强值更高,可使检出限降低。对于50 mg/L羊抗兔IgG样品,流速从130μL/min增至300μL/min时,免疫结合的速度明显加快,可使同一时刻的检出限降低;而样品浓度提高到200 mg/L时,增大流速对免疫反应的促进效果弱于低浓度时的变化。在样品浓度50 mg/L和循环流速300μL/min的条件下,循环给样方式仅需7μL样品溶液就得到了与持续进样约1.8 mL样品溶液基本一致的结果。
A microfluidic chip based circular injection method for bio-samples was presented.The microfluidic chip with an annular peristaltic micropump and electromagnetic microvalves was fabricated using PDMS（polydimethylsiloxane） soft lithography process.The chip was then integrated with an immune probe chip immobilized with rabbit IgG,and realized the circular injection of Goatanti -rabbit IgG（FITC labeled） solution and sample enrichment.Higher fluorescence intensity was obtained using circular injection than that using static adsorption,under different sample concentrations and 3 min binding time.By increasing the pumping speed from 130μL/min to 300μL/min,the immune binding speed with 50 mg/L sample concentration was accelerated evidently,which meant the detection limit at a given time could be lower,but for 200 mg/L sample concentration,the effect was weaker.Compared to the continuous injection mode with about 1.8 mL of sample consumption,only 7μL sample was needed to realize the coincident results with the circular injection mode.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2012年第11期1668-1673,共6页
Chinese Journal of Analytical Chemistry
基金
国家重点基础研究发展计划(No.2007CB310504)
国家自然科学基金(No.50730009)资助项目
关键词
微流体
微泵
循环
给样
富集
Microfluidic
Micropump
Circulation
Sample injection
Enrichment
