摘要
冷杉梢斑螟和赤松梢斑螟常伴随发生,危害红松球果。为探讨利用红松诱导防御抵抗梢斑螟危害的可行性,以不同浓度茉莉酸甲酯、茉莉酮喷雾处理红松,采集健康、虫害及喷雾处理红松的球果和主梢,利用高效液相色谱技术测定其酚酸含量的时序变化。在健康红松球果中检测到香豆酸、苯乙酸、阿魏酸、水杨酸和肉桂酸等5种酚酸,在主梢中检测到香豆酸、苯乙酸、苯甲酸、水杨酸和肉桂酸等5种酚酸,两部位酚酸数量相同但组分存在差异。梢斑螟幼虫取食危害后,球果内出现原儿茶酸和苯酚,主梢内出现苯酚和阿魏酸,且除球果内香豆酸含量下降外,球果和主梢内其余几种酚酸含量均显著增加。相对茉莉酸甲酯,喷施茉莉酮诱导的酚酸种类更多,除原儿茶酸、绿原酸、丁香酸外其余酚酸种类与虫害诱导相同,且诱导时间与虫害取食更为接近。10mmol/L的原位或侧枝喷雾,诱导各酚酸含量即显著高于虫害诱导,可以利用此浓度的茉莉酮喷雾处理增加红松抗性,抵御梢斑螟幼虫取食。
Dioryctria abietella and D. sylvestrella are close relative species, both of which damage the cones concomitantly on Pinus koraiensis. In this study, we investigated the phenolic acid content in the main tips and cones of Korean pine treated by jasmonates sprayed, to explore induce defense of pine. We collected healthy, larvae fed, and jasmonates sprayed tips and cones, to determine phenolic acid content by high performance liquid chromatography （ HPLC）. The results showed that 5 kinds of phenolic acid, including coumaric acid, phenylacetic acid, ferulic acid, salicylic acid and cinnamic acid were detected in healthy cones. Meanwhile, 5 number but different kinds of phenolic acid in healthy main tips were determined, including coumaric acid, phenylacetic acid, benzoic acid, salicylic acid and cinnamic acid. After larvae fed, protocatechuic acid and carbolic acid in cones, and carbolic acid, ferulic acid in tips were detected, and many kinds of phenolic acid （chlorogenic acid, caffeic acid, syringic acid, phenylaetic acid, ferulic acid, benzoic acid, salicylic acid, cinnamic acid）were significantly increased in cones and main tips, whereas coumaric acid decreased in cone compared with healthy plant. Exogenous cis-jasmone induced more kinds of phenolic acid, such as carbolic acid in cones, and syringic acid in both cones and main tips compared with methyl-jasmonate treatments. In addtion, protocatechuic acid, chlorogenic acid, syringic acid, phenolic acids in cones and tips treated by exogenous cis-jasmone were higher than that of herbivore-induced, while the content and induction time of other kinds of phenolic acid by cis-jasmone and herbivore induced were more similar. 10 mmol/L exogenous cis-jasmone point or systematic atomizing sprayed induced phenolic acids were already significantly higher or similar with herbivore-induced. So 10 mmol/L is the effective dose enhancing the resistance of host pine against shoot borer larvae feeding.
出处
《北京林业大学学报》
CAS
CSCD
北大核心
2012年第6期98-106,共9页
Journal of Beijing Forestry University
基金
国家自然科学基金项目(30972375)
黑龙江省杰出青年基金项目(JC200802)
黑龙江省重点科技攻关项目(GA09B203-5)
关键词
红松
茉莉酸甲酯
茉莉酮
酚酸
赤松梢斑螟
冷杉梢斑螟
Pinus koraiensis
methyl-jasmonate
cis-jasmone
phenolic acid
Dioryctria sylvestreUa
D. abietella
