摘要
目的采用日产Olympus AU640酶法与美产Medica EasyLyte Plus Na/K/Cl Analyzer电极法测定血清钾、钠,并对方法学各自的优缺点进行比较。方法参照美国国家临床实验室标准化委员会EP9-A文件,每天随机抽取8份临床标本,分别采用两种方法测定标本的钾、钠离子含量,连续测定10d。记录相关数据,并进行匹配分组、统计学计算。结果本研究中的2台仪器均有较好的精密度(批内及批间变异系数均小于1%)和准确度(回收率为100.25%～101.34%)。在对比试验中,钾、钠离子的含量与患者的年龄和性别没有太大的相关性,2台仪器所测数据的变异系数均较接近。在配对试验中,2台仪器所测得的P值均大于0.05,差异无统计学意义,即黄疸对2台仪器的测定结果均无影响。结论采用全自动生化分析仪与电解质分析仪检测钾、钠离子的准确度较高、精密度良好。钾、钠离子的含量与患者的年龄、性别及标本有无黄疸没有太大的相关性。
Objective To use Japan′s production Olympus AU640 enzymatic and U.S.production Medica in EasyLyte Plus Na/K/Cl Analyzer electrode analysis for serum potassium,sodium methodology,and to compare their respective advantages and shortcomings.Methods According to the national clinical laboratory standardization committee EP9-A file,every random eight clinical specimens were respectively collected for two method for determining contents of potassium and sodium ion,and continuous determination were taken for ten days.The relevant data was recorded and matched group,calculated statistically.Results Two instruments had good precision（batch of variation coefficient between and within all less than 1%） and accuracy（the recovery was 100.25%-101.34%）.In contrast test,the contents of potassium and sodium ions had not related to the patient′s age and gender,two instrument of the coefficient of variation of the test data were closer.In matching tests,the two instrument measured that P value was more than 0.05,there was no significant difference,namely to both instruments,jaundice had no influence on all the determination results.Conclusion Application of automatic biochemistry analyzer and electrolyte analyzer testing K＋,Na＋ has high accuracy and good precision.The contents of K＋,Na＋ are not related to the patient′s age,gender and specimens′ jaundice.
出处
《检验医学与临床》
CAS
2012年第22期2827-2829,共3页
Laboratory Medicine and Clinic
关键词
钾
钠
酶法
电极法
potassium
sodium
enzymatic electrode
