摘要
以25个萝卜品系和组合为试材,采用田间随机区组设计,肉质根采收后采用3,5-二硝基水杨酸法测定萝卜α,β?淀粉酶活性及总酶活性,探讨不同萝卜α,β?淀粉酶活性及总酶活性的差异并建立α,β?淀粉酶及总淀粉酶活性之间的关系。结果表明:萝卜不同品种和组合的α,β?淀粉酶活性及总淀粉酶酶活性淀粉酶活性差异显著,其中A04、S01、A17、A20、S05和A07的α-淀粉酶活性较高;β?淀粉酶活性及总淀粉酶酶活性较高的品系同为A19、A06、A17、A12和S03。α?淀粉酶活性占总酶活性的比例为8.11%～25.57%,β?淀粉酶活性为75.26%～91.89%,三者之间关系为Y总淀粉酶活性=-0.029+1.039Xα-淀粉酶活性+0.998Xβ-淀粉酶活性。
To determine theα,β-amylase activity and total amylase activity in 25radish varieties,field experiments used randomized block design and repeated 3times,α？amylase,β-amylase and total amylase activity were estimated by the 3,5-dinitrosalicylic acid method after fleshy root harvest,for providing a reference and establishment relationship of α？amylase,β-amylase and total amylase activity.The results showed that there were significance differences ofα？amylase,β-amylase and total amylase activity in different radish varieties;the A04,S01,A17,A20,S05and A07were the higher α？amylase activity and the A19,A06,A17,A12and S03were the the higher β-amylase and total amylase activity.The ratio ofα,β-amylase activity in total amylase activity was 8.11%to 25.57% and 75.26%～91.89%,respectively and the relationship of them was Y Total amylase activity =-0.029＋1.039X α-amylase activity ＋0.998X β-amylase activity.
出处
《北方园艺》
CAS
北大核心
2012年第22期21-23,共3页
Northern Horticulture
基金
河北省教育厅自然科学基金资助项目(Z2009303)
河北北方学院资助项目(2001002)
关键词
萝卜
淀粉酶
品种
Raphanus sativus L.（radish）
amylase activity
variety
