摘要
为了初步了解"福白菊"与其它杭系菊花栽培品种亲缘关系,以不同产地药用菊花的叶片做POD同工酶分析,并运用相似系数法进行亲缘关系的聚类分析,从谱带分布和酶谱距离判定不同种间POD同工酶谱间的差异。结果表明:营养生长期,叶比茎的POD酶带更清晰,更整齐,分离更好,营养生长阶段的叶最适合用于菊花POD同工酶分析;"杭黄菊"、"金菊2号"、"金菊1号"、"杭小洋菊"4个品种间亲缘关系极近。"杭白菊"和"射阳大白菊"相似系数为0.89,可归为1类。"福白菊"、"黄山贡菊黄花"、"杭迷你小酒菊"亲缘关系较远的都可各自归为一类,尤其是"杭迷你小酒菊"与其它8个菊花品种的亲缘关系较远。该研究可为药用菊花栽培品种的亲缘关系鉴别提供参考,并为药用菊花的分类、遗传育种、资源综合利用提供理论依据。
In order to find out the genetic diversity of Chrysanthemum ＇Fubaiju＇ and provide evidence for evaluation and exploitation of Chrysanthemum germplasm,peroxidase isozyme analysis of 9 germplasm from different habitats were analyzed by using polyacrylamide gel electrophoresis（PAGE）.POD isozyme patterns varied greatly among the species and varieties that studied by anglicizing their band distribute and zymogram distance.The results showed that there were more clear POD isozyme bands in leaf than stem.The leaf during vegetative growth stage was the best for POD research;the consanguinity of Chrysanthemum ＇Hanghuangju＇,＇Jinju（number 1）＇,＇Jinju（number 2）＇ and ＇Hangxiaoyangju＇ were very close,＇Sheyangdabaiju＇ and ＇Hangbaiju＇ could return to a species.The consanguinity of Chrysanthemum ＇Fubaiju＇,＇Huangshangongjuhuanghua＇ and ＇Hangminixiaojiuju＇ were far to others,especially ＇Hangminixiaojiuju＇ was very far to others.These study could be used as cultivar identification and protection on biochemical levels.
出处
《北方园艺》
CAS
北大核心
2012年第22期99-103,共5页
Northern Horticulture
基金
湖北省自然科学基金资助项目(2010CDZ026)
湖北省教育厅高校青年教师深入企业行动计划资助项目(XD2010398)
关键词
“福白菊”
药用菊花
POD同工酶
遗传多样性
Chrysanthemum ＇Fubaiju＇
medicinal Chrysanthemum
POD isozyme
genetic diversity
