摘要
为确定巨大革耳种质资源间的亲缘关系,本文应用ISSR分子标记技术,对来源不同地区的野生或栽培的9个巨大革耳菌株进行遗传多样性分析。从20个引物筛选获得4个ISSR多态性引物对巨大革耳菌株扩增,获得23条多态性条带,多态性比率为85.19%;对扩增结果进行聚类分析,当遗传距离为20%时,9个菌株聚为2类:Ι类包括C.m0002菌株;Π类包括其它的8个菌株。其中C.m0002菌株与其它8个菌株的遗传距离很远。经栽培出菇实验,结果表明,C.m0002菌株的子实体似多脂鳞伞(黄伞),是同名异种;其它8个菌株均为巨大革耳。ISSR分析的结果与子实体形态特征一致。
In order to evaluate the genetic relationship among germplasm of Panus giganteus, the genetic diversity of 9 P. giganteus strains from different regions of china was analyzed using ISSR markers. 4 ISSR primers were selected from 20 primers were applied to amplify the DNA of 9 strains. The results showed that polymorphic bands were 23, and the poly- morphic rates were 85.19%. UPDMA dendrogram based on ISSR markers indicated that 9 P. giganteus strains could be distinguished into 2 groups when genetic distance was 20%, and the I group included C.m0002 strain, the group II in- cluded the other 8 strains. The genetic distance between C.m0002 strain and the other 8 strains was large. The strain C. m0002 might be Phonliota adipose, and the other 8 strains were Panus giganteus by cultivation experiment. Their genetic relation based on ISSR markers was corresponded with the one based on fruit body traits.
出处
《中国食用菌》
2012年第6期32-34,共3页
Edible Fungi of China
基金
福建省科技厅项目(08SZ00021)
国家星火计划项目(2011GA720008)
