摘要
根据查尔酮合成酶(CHS)基因DNA序列的保守区域设计了PCR引物,通过RT-PCR扩增从大豆叶片中克隆出3个参与类黄酮合成的CHS基因,分别命名为GmCHS1、GmCHS2和GmCHS3。在大豆基因组数据库进行同源比对,发现这3个基因分别与大豆基因组上Gm08g11610、Gm05g28610和Gm08g11520相对应,DNA序列一致性达95%～98%,推导氨基酸序列一致性达98%以上。进化分析显示,大豆中3个CHS蛋白与决明、菜豆CHS蛋白亲缘关系较近。表达分析显示,这3个基因在不同品种间有表达水平的差异,这可能是不同大豆品种中类黄酮含量不同的重要原因之一。
In order to clarify the biosynthesis of flavonoids in soybean seeds and to know the reason for fla- vonoids content variation among different soybean lines, PCR primers designed from the conservative do- main of Chalcone Synthase （CHS） were used to isolate the CHS genes from soybean leaves by RT-PCR. As the results,three CHS genes involved in the biosynthesis of fiavonoids were cloned out and named as GrnCHS1,GmCHS2 and GmCHS3. After BLAST search against the soybean genome sequencing data base, the three CHS genes are identical with Gm08gl1610,GmOSg28610 and Gm08gl1520, respectively. Se- quences alignment results indicated these three CHS genes shared 95%-98% identity on DNA sequence, and 98%identity on amino acids sequence. 6 amino acids difference was found among these three CHS. Phylogenic analysis on the amino acid sequences of CHS from soybean and some other plants showed that the three soybean CHS were closely related to Senna tora and Phaseolus vulgaris. Gene expression analysis showed that the three CHS genes were differently expressed between two lines, which may be one of the major reasons leads to the variation of flavonoids contents in seeds among different soybean lines.
出处
《西北植物学报》
CAS
CSCD
北大核心
2012年第11期2164-2168,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
西北农林科技大学引进人才科研启动基金
三秦学者基金
