摘要
本文建立了用反相液相色谱同时测定糖品中的苯甲酸、山梨酸、脱氢乙酸、安赛蜜、糖精钠等防腐剂和甜味剂的方法。方法采用Venusil XBP-C18(5μm,4.6×250mm)反相色谱柱,流动相为20mmol/L乙酸铵-甲醇,流速为1.0mL/min,柱温为30℃,进样量为10μL。该方法的测定低限为1.0 mg/L,线性范围为10～100mg/L,加标回收率为89.85%～103.60%,相对标准偏差为1.50%～3.26%(n=4)。结果表明,该方法简便、可靠,适合对糖品中防腐剂和甜味剂的同时检测。
Reverse-phase HPLC was applied to the determination of benzoic acid, sorbic acid, dehydroacetic acid, acesulfame K, sodium saccharin in sugar. The analysis column was Venusil XBP - C18 （5 μm, 4.6×250 mm）, the mobile phase was Methanol - 20 mmol/L NHgAc, the flow rate was 1.0 mL/min. The column temperature was 30 ℃, and the injection volume was 10 μL. The limit of quantitation was 1.0 mg/L. The linear plots were obtained between 10 and 100 mg/L. Overall recoveries were between 89.85% and 103.60%, and RSD （n = 4） value were 1.50% and 3.26%. Results showed this method was simple and accurate, it could be applied for determination of preservative and sweetener in sugar.
出处
《甘蔗糖业》
2012年第5期58-62,共5页
Sugarcane and Canesugar
基金
广东省科技计划项目(2011B040300001)
