摘要
采用PCR和Western-blot方法,研究了储藏过程中不同温度(4、25和37℃)和不同时间(10、30、50、70和90 d)对转基因大豆外源cp4-epsps基因和EPSPS蛋白的影响。结果表明:在储藏过程中外源cp4-epsps基因没有发生明显变化;外源EP-SPS蛋白发生轻微降解,降解产物相对分子质量约为46.2×103,而且37℃降解条带较25℃明显,说明相对较高的温度(25和37℃)在储藏90 d期间能够促进外源EPSPS蛋白的降解,而对基因完整性影响较小。
In this study,the effect of different storage temperatures（4,25,37 ℃）and times（10,30,50,70,90 d）on exogenous cp4-epsps gene and protein of genetically modified soybean was investigated by PCR and Western-blot methods.The results suggested that there were no obvious changes in nucleic acid during the storage periods.However,the degradation product（relative molecular mass 46.2×103）of EPSPS protein was detected at 25 ℃ and 37 ℃.The conclusion revealed that the storage temperature and time can promote the degradation of exogenous EPSPS protein,but exert no effect on integrity of the exogenous cp4-epsps gene during the storage period（90 days）.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2012年第6期131-136,共6页
Journal of Nanjing Agricultural University
基金
南京市生物农业项目(转基因农产品检测试剂盒研究与开发)
转基因生物新品种培育重大专项(2009ZX08012-014B)
