摘要
通过对浸提温度、浸提时间、液料比和醇沉浓度的考察,确定了平菇中多糖的最佳提取工艺,在此基础上,对实验室六种培养基、两个生产周期的平菇进行了多糖含量变化的研究。结果表明:最佳浸提温度为90℃、浸提时间3 h、液料比30︰1、醇沉浓度90%;平菇中多糖的含量随培养基中棉籽壳与秸秆的比例的增大而增大;随着生产周期的延长、培养基的营养成分减少,平菇中多糖的含量也随之降低;与市售平菇相比,实验室2号培养基、第二周期培养出的平菇,其多糖含量也比市售的高,说明实验室培养基种植的平菇其多糖含量更丰富。
Extraction temperature, extraction time, ratio of solvent to material and alcohol sedimentation concentration have been investigated to determine the optimum extraction process of polysaccharides from pleurotus ostreatus. On this basis, the change of polysaccharide content of six kinds of medium, two periods was studied. The results showed that, the best extraction temperature was 90 ~C, extraction time was 3 h, ratio of solvent to material was 30 ： 1, alcohol sedimentation concentration was 90 %. The polysaccharide content was positive related to the ratio of cottonseed hull content to straw content. With the extension of the production period, the polysaccharide content also showed a decreasing rate, following the decrease of medium nutritional conditions. Compared with the commercial pleurotus ostreatus, the polysaccharide content of No.2 medium was still much higher.
出处
《广东化工》
CAS
2012年第15期99-100,58,共3页
Guangdong Chemical Industry
基金
安徽省教育厅自然科学重点项目KJ2012A249资助
合肥学院院级科研项目12RC05资助
国家级生物工程特色专业建设点资助
关键词
平菇
多糖
水提醇沉
培养基
pleurotus ostreatus
polysaccharide
water extraction and alcohol precipitation
medium
