摘要
目的:探讨参附注射液对局灶性脑缺血大鼠血脑屏障(blood brain barrier,BBB)的影响及其机制。方法:利用线栓法制作脑缺血/再灌注模型,再灌注24h后进行神经功能评分,应用伊文思蓝法(Evans Blue,EB)观察对照组与参附注射液治疗组(SFI)BBB通透性,采用干湿重法比较脑含水量、TTC法比较脑梗死面积。蛋白印迹法检测金属硫蛋白酶9(matrix metalloproteinase-9,MMP-9)及细胞外调节蛋白激酶(Extracellular regulated protein kinases,ERK)的表达。结果:参附注射液治疗组的血脑屏障通透性、脑含水量、脑梗塞体积、神经功能缺损程度均比对照组明显降低(P<0.05)。和对照组相比,SFI组的MMP-9及磷酸化ERK的蛋白表达明显降低。结论:参附注射液对大鼠脑缺血模型的血脑屏障具有保护作用,其作用机制与抑制MMP-9及ERK信号通路相关。
Objective ： To investigate the effect of Shenfu injection on focal cerebral isehemic stroke blood - brain barrier （blood brain barrier, BBB） in rats and its mechanism. Methods： An ischemic stroke model was generated by 90 rain occlusion of right middle cerebral artery. After 24h reperfusion, neurological score was evaluated, the cerebral infarct volumes were quantified by 2,3,4 -triphenytetrazolium -chloride （TTC）, brain water content was determined by dry/ wet weight method, and damage to the blood - brain barrier （BBB） was determined by Evans blue extravasation. Metal- lothionein protease （the matrix metalloproteinase -9, MMP -9） and extraeellular regulated protein kinase （Extracellu- lar regulated protein kinases, ERK） expression were evaluated by Western blot. Results ： Shenfu Injection - treatment group （SFI） significantly reduced infarct volume, brain edema, blood - brain barrier permeability, the degree of neuro- logical deficits （P 〈 0.05 ）. And compared with the control group, the protein expression of MMP - 9 and phosphoryla- tion of ERK were significantly reduced in the SFI group. Conclusion ： Shenfu injection plays a neuroprotective role in the blood- brain barrier in the experimental cerebral ischemia model. The mechanism is associated with the inhibition of MMP -9 and ERK signaling pathway.
出处
《中医药学报》
CAS
2012年第5期76-79,共4页
Acta Chinese Medicine and Pharmacology
基金
国家自然科学基金项目(81171241)
北京市优秀人才D类资助(2011D005018000007)
关键词
参附注射液
缺血性脑卒中
血脑屏障
金属硫蛋白酶9
细胞外调节蛋白激酶
Shenfu injection
Ischemic stroke
blood - brain barrier
Matrix metalloproteinase - 9
Extracellular regulatedprotein kinases
