摘要
目的:建立高效液相法测定婴儿止咳合剂中桔梗皂苷D含量的方法。方法:以甲醇溶液提取婴儿止咳合剂中的桔梗皂苷D,采用十八烷基硅烷键合硅胶色谱柱;流动相为乙腈-水(25∶75),流速0.5mL·min-1,蒸发光散射检测器检测,波长210nm,柱温为30℃。结果:RP-HPLC-ELSD的平均加样回收率为98.99%,RSD%为0.37%(n=6)结论:该方法简便易行,准确可靠,可用于婴儿止咳合剂的质量控制。
Objective： To establish a method for the determination of the platycodin D in Ying＇er Zhike Mixture using high performance liquid chromatography-evaporative light-scattering detector （RP-HPLC-ELSD）. Method： The RP-HPLC-ELSD separation was carried out on a Cls column with acetonitrile-water （25：75） as mobile phase at a flow rate of 0. 5 mL.min-1 at 30℃ , and detected at 210 nm. Result： The mean recovery of platycodin D was 98.99% , and the relative standard deviation （ RSD ） was 0.37% （ n = 6 ）. Conclusion： The method is convenient, accurate and trustworthy, and could be used for quality control of the Ying＇er Zhike Mixture.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第23期130-132,共3页
Chinese Journal of Experimental Traditional Medical Formulae
