摘要
目的建立同时测定梅核气胶囊中绿原酸和柚皮苷含量的方法。方法采用HPLC法。色谱柱:Shim-pack VP-ODS;柱温:30℃;流动相:乙腈-0.4%磷酸溶液梯度洗脱;流速:1.0mL.min-1;检测波长:283nm;进样量:20μL。结果绿原酸和柚皮苷分别在14.4～108.0μg.mL-1(r=0.9999)和6.0～45.0μg.mL-1(r=0.9999)范围内与峰面积呈良好线性关系;平均加样回收率分别为99.65%(RSD=1.02%,n=9)和99.60%(RSD=1.13%,n=9)。结论本法简便、结果准确、重复性好,可用于梅核气胶囊的质量控制。
Objective To develop an HPLC method for the determination of chlorogenic acid and naringin in Meiheqi Jiaonang.Methods Analysis were carried out on VP-ODS column with acetonitrile-0.4% phosph acid（gradient elution）as the mobile phase.The flow rate was 1.0 mL·min-1 and the UV detection wavelength was set at 283 nm.Results The linearities of chlorogenic acid and naringin were in the range of 14.4-108.0 μg·mL-1（r=0.9999）and 6.0-45.0 μg·mL-1（r=0.9999）,respectively.The average recoveries of chlorogenic acid and naringin were 99.65% and 99.60%,the RSD were 1.02% and 1.13%.Conclusion The method is simple,accurate,highly specific and can be used for the quality control of Meheqi Jiaonang.
出处
《中国药事》
CAS
2012年第10期1115-1118,共4页
Chinese Pharmaceutical Affairs
基金
天津市自然科学基金(编号11JGYBJC14300)
