摘要
目的:建立生津益气口服液的质量标准。方法:采用薄层色谱法对生津益气口服液合剂中的黄芪、五味子、黄连、赤芍进行鉴别,采用HPLC-ELSD法测定黄芪甲苷的含量,色谱柱为Agilent C18柱,流动相为乙腈-水(32∶68),流速为1.0 ml·min-1,进样量为10μL,柱温为35℃,ELSD检测器漂移管温度为100℃,载气流量为2.7 L.min-1。结果:在TLC色谱中黄芪、五味子、黄连、赤芍均能检出;黄芪甲苷在0.11~0.90 mg·ml-1范围内呈良好的线性关系,r=0.999 3。平均加样回收率为97.3%,RSD为1.13%(n=6)。结论:该法简便,准确,专属性强,能有效控制生津益气口服液的质量。
Objective: To establish the quality standard for Shengjin Yiqi oral liquids.Method: Astragali Radix,Schisandrae Chinensis Fructus,Coptidis Rhizoma and Paeohiae Radix Rubra were identified by TLC,and the content of astragaloside IV was determined by HPLC-ELSD.Result: Astragali Radix,Schisandrae Chinensis Fructus,Coptidis Rhizoma and Paeohiae Radix Rubra were all detected by TLC.Astragaloside IV showed a good linear relationship within the range of 0.11-0.90 mg·ml-1(r=0.999 3).The average recovery was 97.3% and RSD was 1.13%(n=6).Conclusion: The method is simple and accurate with good specificity,and can control the quality of Shengjin Yiqi oral liquids effectively.
出处
《中国药师》
CAS
2012年第10期1418-1420,共3页
China Pharmacist