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Preparation and Identification of Monoclonal Antibodies against Salbutamol

Preparation and Identification of Monoclonal Antibodies against Salbutamol
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摘要 [ Objective] To prepare specific monoclonal antibodies against salbutamol (SAL) and thus to provide the basis for the immunological detection. [Method] Balb/c mice were immunized with SAL-BSA. Hybridoma strains (SAL rnAb) secreting monoclonal antibody against SAL were established through cell fusion. Ascites of SAL mAb were prepared in vivo and the immunological characteristics were observed. [Resalt] Two hybridoma strains, SAL-2D9 and SAL-1C4, were established, and the indirect ELISA titers of the cell supematant and ascites were 1:1 × 10^4 and 1 : 1 × 10^6, respectively. Their isotype was IgGl. The median inhibitory concentration (IC5o) of the SAL mAb against SAL was 1.14 ng/ml. It had cross-reactivity of 100% against SAL and 26.09% against clenobuterol hydrochloride, and no cross-reaction was found between the SAL mAb and other SAL analogues. [ Conclusion] The high-titer, sensitive and specific anti-SAL monoclonal antibodies have been produced, which makes it possible to develop immunoassay for detection of SAL residues. [ Objective] To prepare specific monoclonal antibodies against salbutamol (SAL) and thus to provide the basis for the immunological detection. [Method] Balb/c mice were immunized with SAL-BSA. Hybridoma strains (SAL rnAb) secreting monoclonal antibody against SAL were established through cell fusion. Ascites of SAL mAb were prepared in vivo and the immunological characteristics were observed. [Resalt] Two hybridoma strains, SAL-2D9 and SAL-1C4, were established, and the indirect ELISA titers of the cell supematant and ascites were 1:1 × 10^4 and 1 : 1 × 10^6, respectively. Their isotype was IgGl. The median inhibitory concentration (IC5o) of the SAL mAb against SAL was 1.14 ng/ml. It had cross-reactivity of 100% against SAL and 26.09% against clenobuterol hydrochloride, and no cross-reaction was found between the SAL mAb and other SAL analogues. [ Conclusion] The high-titer, sensitive and specific anti-SAL monoclonal antibodies have been produced, which makes it possible to develop immunoassay for detection of SAL residues.
出处 《Animal Husbandry and Feed Science》 CAS 2011年第1期33-34,38,共3页 动物与饲料科学(英文版)
基金 funded by the Key Technology R&D Program of Hebei Province (09227134D) Key Technology R&D Program of Shijiazhuang City (10150142A)
关键词 Salbutamo Hybddomas Monoclonal antibody Salbutamo Hybddomas Monoclonal antibody
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