摘要
[ Objective] To isolate fetal fibroblasts from Tibetan yellow cattle and thus to provide the ideal biological material for cloning and cellular biology of animals inhabiting in the Tibet Plateau. [ Method] The fibroblast cells were isolated from tissues of Tibetan yellow fetus by explant culture method. The subculture, morphology and dynamics were observed. [ Result] The fetal fibroblasts of Tibetan yellow cattle had typical morphology of fibroblast calls. The isolated cells had three growth stages including retention period （0 -2 d）, logarithmic phase （2 -7 d） and platform phase （7 -8 d）. Its doubling time was 60 h. [ Conclusion] Fetal fibroblasts of Tibetan yellow cattle have been successfully cultured.
[ Objective] To isolate fetal fibroblasts from Tibetan yellow cattle and thus to provide the ideal biological material for cloning and cellular biology of animals inhabiting in the Tibet Plateau. [ Method] The fibroblast cells were isolated from tissues of Tibetan yellow fetus by explant culture method. The subculture, morphology and dynamics were observed. [ Result] The fetal fibroblasts of Tibetan yellow cattle had typical morphology of fibroblast calls. The isolated cells had three growth stages including retention period （0 -2 d）, logarithmic phase （2 -7 d） and platform phase （7 -8 d）. Its doubling time was 60 h. [ Conclusion] Fetal fibroblasts of Tibetan yellow cattle have been successfully cultured.
基金
supported by National Natural Science Foundation of China (31040077)
University Teachers Team Construction Program of"211 Project"(SZRC-211-01)
Regional Natural Science Foundation of Tibet Science and Technology Department (2010-17-02)
