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Intraspecific Relationship of Sheep Based on Mitochondrial Cytochrome b Gene

Intraspecific Relationship of Sheep Based on Mitochondrial Cytochrome b Gene
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摘要 [ Objective] To preliminarily explore the intraspecific relationship of sheep based on cytochrome b ( Cyt b) gene of mitochondrial DNA (mtDNA). [Method] The Cyt b gene sequences in 112 sheep individuals of two local sheep breeds were amplified by PCR. Then the amplified products were digested with EcoR I and analyzed by restriction fragment length polymorphism (RFLP). [ Result] As many as 56 samples from Tan sheep and 56 samples from Wadi sheep were detected. The results showed that the amplified Cyt b gene in 51 individuals of Tan sheep had one EcoR I restriction site and no EcoR I restriction site in other five individuals, thus the Cyt b gene in Tan sheep showed two restriction morphs; the Cyt b gene in all 56 individuals of Wadi sheep had one EcoR I restriction site and showed one restriction morph. [ Conclusion] The polymorphism of mitochondrial Cyt b gene in Tan sheep and Wadi sheep is poor, and the Cyt b gene in sheep breeds is very conservative. Therefore, using Cyt b gene as gene marker to study the intraspecific relationship of sheep has some limitations. [ Objective] To preliminarily explore the intraspecific relationship of sheep based on cytochrome b ( Cyt b) gene of mitochondrial DNA (mtDNA). [Method] The Cyt b gene sequences in 112 sheep individuals of two local sheep breeds were amplified by PCR. Then the amplified products were digested with EcoR I and analyzed by restriction fragment length polymorphism (RFLP). [ Result] As many as 56 samples from Tan sheep and 56 samples from Wadi sheep were detected. The results showed that the amplified Cyt b gene in 51 individuals of Tan sheep had one EcoR I restriction site and no EcoR I restriction site in other five individuals, thus the Cyt b gene in Tan sheep showed two restriction morphs; the Cyt b gene in all 56 individuals of Wadi sheep had one EcoR I restriction site and showed one restriction morph. [ Conclusion] The polymorphism of mitochondrial Cyt b gene in Tan sheep and Wadi sheep is poor, and the Cyt b gene in sheep breeds is very conservative. Therefore, using Cyt b gene as gene marker to study the intraspecific relationship of sheep has some limitations.
出处 《Animal Husbandry and Feed Science》 CAS 2010年第6期14-16,共3页 动物与饲料科学(英文版)
基金 supported by the National Science and Technology Support Planning Project (2008BADB2B04-8-4) Natural Science Foundation of Hebei Province (C2008001308)
关键词 SHEEP Cytochrome b gene Intraspecific relationship Sheep Cytochrome b gene Intraspecific relationship
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