摘要
试验所用的野生蓝靛果果实采摘于伊春市五营林业局,其具有非常好的营养价值和生物学活性,且野生资源稀少、产量很低,是一种很有开发潜力的野生浆果植物品种。该项试验以野生蓝靛果红色素质量浓度为指标,研究了五种HPD系列大孔吸附树脂纯化蓝靛果红色素的吸附及洗脱条件,筛选分离纯化了蓝靛果红色素的最佳树脂,并得到了优化的分离纯化工艺。结果表明,HPD-200型大孔吸附树脂为分离纯化蓝靛果红色素的最佳树脂。其纯化工艺条件为:pH为3.0,一样质量浓度为3.0g/L,上样流速为1.0mL/min;洗脱剂为50%乙醇,洗脱流速为1.5mL/min。精制后的蓝靛果色素的色价比粗制品提高了11倍,色素得率为87.6%。该分析结果对蓝靛果的食用、药用、染料及化妆品等方面的利用研究开发具有科学价值。
The wild indigo fruit used in the test was picked in wuying forestry bureau in Yichun city.It has good nutrition and biological activity.The natural resource of the fruit is very limited.Therefore,it has the potential of development. In order to select the best resin to purify red pigment of Lonicera edulis and obtain the optimized separation and purification parameters,the adsorption and elution conditions of five HPD series adsorption resins were studied.This paper used mass concentration of Lonicera edulis red pigment as the index.The results shows that HPD - 200 macroporous adsorption resin was the best to separate and purify red pigment of Lonicera edulis.The optimum purification conditions were;the pH value of absorbing solution 3.0,the concentration of sample 3.0g/L,the flow rate of sample 1.0mL/min,the eluent ethanol concentration was 50%,and the flow rate of eluent was 1.5mL/min.After refining, the color value was increased more than 11 times,pigment yield was 87.6%.The experiment provides scientific view in the research and development of Lonicera edulis red pigment on edible,medical,dye and cosmetics areas.
出处
《中国食品添加剂》
CAS
北大核心
2012年第5期154-158,共5页
China Food Additives
基金
黑龙江省中医药管理局科技项目(ZHY12-W041)
佳木斯大学科技项目(S2011-055)
黑龙江省基金项目(C201102)
关键词
蓝靛果
吸附树脂
分离纯化
工艺
lonicera edulis
adsorption resins
separation and purification
craft
