摘要
目的探讨不同方法对制备的富血小板纤维蛋白(platelet-rich fibrin,PRF)释放生长因子的影响。方法对志愿者进行静脉采血,分别以不同离心速度(2500r/min、3000r/min、3500r/min)、离心时间(10min、15min、20min)和除水方法(快速除水和缓慢除水)制备PRF,收集PRF所释放的生长因子,通过ELISA法比较不同方法所获得的PRF释放的TGF-β和PDGF-AB的浓度差异。结果以3000r/min的离心速度制备的PRF释放TGF-β和PDGF-AB含量显著高于其他两组;10min组和15min组制备的PRF释放TGF-β含量高于20min组,而PDGF-AB含量10min组显著高于其他两组;两种除水方法制备的PRF释放TGF-β和PDGF-AB含量无统计学差异(P>0.05)。结论不同离心速度、离心时间对PRF的特性存在一定的影响,而快速除水和缓慢除水对PRF特性的影响无显著性差异。
Objective %To investigate the influence factors of platelet-rich fibrin releasing growth factors with different kinds.Methods PRF were produced by different centrifugal speed（2500r/min、3000r/min、3500r/min）, centrifugal time（10min、15min、20min） and dehydration methods（fast dehydration methods and slow dehydration methods） from volunteers.Collect the growth factors in PRF,and Compare the TGF-β and PDGF-AB concentration by ELISA.Results TGF-βand PDGF-AB levels in the PRF prepared by 3000r/min were higher;TGF-βlevel in the PRF prepared by 10min and 15min were higher,and PDGF-AB level in the PRF prepared by 10min were higher;but the two dehydration methods showed no statistics significance（P0.05）.Conclusion Different centrifugal speed and centrifugal time may affect the characteristics of PRF,and dehydration methods may not affect the characteristics of PRF.
出处
《现代口腔医学杂志》
CAS
CSCD
2012年第6期404-407,共4页
Journal of Modern Stomatology
基金
吉林省科技厅科研课题(2012-5053)
