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缺氧诱导因子1在创伤性深静脉兔模型血栓形成及消退过程中的表达(英文) 被引量:1

Hypoxia-inducible factor-1 expression in thrombosis and thrombolysis of a rabbit model of traumatic deep vein thrombosis
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摘要 背景:深静脉血栓形成及消退过程中的分子机制极其复杂,目前已有研究表明,缺氧与损伤因素参与了深静脉血栓形成及消退过程。目的:观察静脉壁中缺氧诱导因子1在创伤性深静脉血栓模型兔血栓形成及消退过程中的表达变化。方法:将日本大耳白兔采用钳夹双侧股静脉+石膏固定双下肢建立兔创伤性深静脉血栓模型。PCR及ELISA检测股静脉组织中缺氧诱导因子1mRNA、蛋白在兔创伤性深静脉血栓建模后表达的变化。结果与结论:模型兔创伤后24h,经B超监测,血栓形成率为58%;血栓栓子随造模后时间延长逐渐缩小机化,创伤后第5天开始经B超监测模型组血栓形成的血管腔有断续血流信号。模型兔股静脉组织中缺氧诱导因子1在造模后1,3,5d表达逐渐升高(P<0.05或P<0.01),此后7-14d表达逐渐下降(P<0.05或P<0.01)。结果证实,在创伤性深静脉血栓形成过程,缺氧诱导因子1表达上调,在血栓形成后的溶解及机化再通过程中,缺氧诱导因子1表达下调。 BACKGROUND:The molecular mechanism of deep vein thrombosis and further regression is extremely complex.Researches show that hypoxia and injury factors are involved in deep vein thrombosis and regression.OBJECTIVE:To investigate changes in hypoxia inducible factor(HIF)-1 expression during deep vein thrombosis and regression in a rabbit model of traumatic deep vein thrombosis(TDVT).METHODS:The bilateral femoral vein of rabbits was clamped and both lower extremities were fixed with gypsum to establish model of TDVT.SYBR Green I fluorescence real-time quantitative PCR and ELISA dynamic detection were used to determine the change of HIF-1 mRNA and protein expression in femoral vein in the rabbits with TDVT.RESULTS AND CONCLUSION:At 24 hours post-injury,B-ultrasonography showed that thrombosis rate was 58% and thromboembolus gradually reduced and be organized with time;from the 5 th day intermittent flow signals were monitored in lumen of blood vessel with thrombosis of model group.HIF-1 expression in vein of model group gradually increased(P0.05 or P0.01),and reduced between 7-14 days(P0.05 or P0.01).Those findings indicate that HIF-1 expression is upregulated during TDVT,but downregulated during thrombolysis and organization.
出处 《中国组织工程研究》 CAS CSCD 2012年第42期7929-7932,共4页 Chinese Journal of Tissue Engineering Research
基金 the National Natural Science Foundation of China,No.81060151 the Science and Technology Development Program of Yunnan Province,No.2010BC010~~
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