摘要
目的:建立一个较理想的CCl4药物性肝损伤体外模型。方法:分别采用传统方法和改进方法配制CCl4损伤液并诱导人肝HepG2细胞损伤,倒置相差显微镜观察细胞形态学变化,生化法检测上清液中ALT水平并采用MTT法测定细胞活性。结果:改进方法的CCl4诱导损伤效果明显优于传统方法,随着CCl4损伤液浓度的提高,上清液中ALT水平明显升高,而细胞活性显著降低,70%浓度CCl4损伤液诱导损伤4 h可获得最佳损伤效果。结论:利用改进方法可在体外实验中建立较理想的CCl4药物性肝损伤模型,此模型可为进一步的体外实验研究奠定基础。
Objective： To establish an ideal CCl4 drug-induced liver injury model in vitro. Method： Traditional method and improved method were adopted for preparing CCl4 injury liquid and drug-induced human liver HepG2 cell injury. Cell morphological change was observed under a bright-field microscope. The level of alanine aminotransferase （ALT） in supernatant was detected by bio- chemical method. 4-Methyl-tetrazolium （MTT） chromatometry was adopted for determining cell activity. Result： The improved method showed better CCl4-induced injury effect than the traditional method. With the increase in the concentration of CCl4 injury liquid, the ALT level significantly increased, whereas the cell activity notably decreased. Particularly, 70% CCl4 injury liquid use for 4 hours could achieve the best injury effect. Conclusion： The improved method could be used to establish an ideal CCl4 drug-induced liver injury model in vitro, which can lay foundation for further in vitro studies.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第23期3633-3636,共4页
China Journal of Chinese Materia Medica
