摘要
目的研究合肥市男男性行为人群(MSM)中未经抗病毒治疗的人类免疫缺陷病毒Ⅰ型(HIV-1)感染者的HIV-1基因亚型和耐药突变的发生流行情况。方法收集合肥市2011-2012年确认的35例HIV-l感染者外周静脉血,提取血浆中HIV基因组RNA,用逆转录PCR(RT-PCR)和巢式PCR方法扩增HIV-1 pol区核酸序列,基因序列测定、拼接后,上传至美国斯坦福大学HIV耐药数据库在线进行耐药性分析。结果获得的35个pol区有效序列中,CRF01_AE亚型比例最大,达57.1%(20例);其次为B亚型,达34.3%(12例)。没有针对蛋白酶抑制剂(PIs)的主要耐药性基因突变。发现针对非核苷类逆转录酶抑制剂(NNRTIs)的耐药突变2例,其中仅1例携带V179D原发耐药变异。对依非韦伦(efavii-rens,EFV)、奈韦拉平(nevirapine,NVP)、依曲韦林(etravirine,ETR)和利匹韦林(rilpivirin,RPV10)存在不同程度的耐药,RT区耐药株传播率为2.9%。结论在合肥市MSM人群中,未经治疗的新发HIV感染者已经出现针对非核苷类逆转录酶抑制剂(NNRTIs)的耐药突变株,应引起重视;原发耐药目前尚处于较低水平(<5%)。
Objective To analyze the prevalence of drug-resistant HIV-1 strains in the MSM（men who have sex with men）population with recent HIV infections in Hefei city of China.Methods A total of 35 blood samples from MSM population verified with HIV-1 infection were collected and RNA was extracted from the plasma and then the HIV-1 pol gene from 32 samples was amplified by RT-nest-PCR and sequenced.The HIV-1 drug-resistant nucleic acid sites from these isolates were analyzed using the Stanford HIV Drug Resistance Database.Results Among these sequenced strains,CRF01_AE（57.1%）and B（34.3%）were the main circulating HIV-1 subtypes;no PIs resistant strain was found;two NNRTI resistant strains were confirmed and the prevalence rate was 2.9%.Conclusions Primary drug resistant HIV-1 strains were not common in the MSM population of Hefei city of China.
出处
《中国病毒病杂志》
CAS
2012年第6期450-454,共5页
Chinese Journal of Viral Diseases
关键词
人类免疫缺陷病毒Ⅰ型
原发耐药
男男性行为者
Human immunodeficiency virus-1
Primary drug resistance
Men who have sex with men（MSM）
