摘要
Hydroxyapatite(HA) based materials have been widely used in the field of ligament tissue engineering in the past decades.It has been previously reported that HA can increase the penetration of marrow-derived mesenchymal stem cells(MSCs) and MSCs cells into scaffolds due to increased cell differentiation in biological media.Additionally,it was found that there are much difference between MSCs and anterior cruciate ligament (ACL) cells.For that reason,we mainly evaluate the biocompatibility of polyethylene terephthalate(PET) silk scaffold with fibroblasts cells in vitro.We cultured mouse fibroblasts cells on the substrate of PET fiber and PET-HA scaffold,respectively,and then observed the morphology by using scanning electron microscopy.Our data indicate that PET-HA scaffold has good biocompatibility with fibroblasts cells and can potentially be useful in enhancing the fibroblasts cell differentiation and proliferation.
Hydroxyapatite （HA） based materials have been widely used in the field of ligament tissue engineering in the past decades. It has been previously reported that HA can increase the penetration of marrow-derived mesenchymal stem cells （MSCs） and MSCs cells into scaffolds due to increased cell differentiation in biological media. Additionally, it was found that there are much difference between MSCs and anterior cruciate ligament （ACL） cells. For that reason, we mainly evaluate the biocompatibility of polyethylene terephthalate （PET） silk scaffold with fibroblasts cells in vitro. We cultured mouse fibroblasts cells on the substrate of PET fiber and PET-HA scaffold, respectively, and then observed the morphology by using scanning electron microscopy. Our data indicate that PET-HA scaffold has good biocompatibility with fibroblasts cells and can potentially be useful in enhancing the fibroblasts cell differentiation and proliferation.
基金
the Young Project of National Natural Science Foundation of China(No.81000816)
the National Basic Research Program(973) of China (No.2009CB930000)
the Project of Shanghai Municipal Science and Technology Commission (No.11JC1401700)
