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正常人T淋巴细胞亚群端粒酶活性的观察

STUDY ON TELOMERASE ACTIVITY IN HUMAN T LYMPHOCYTES
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摘要 目的:探讨正常人外周血T淋巴细胞亚群端粒酶活性表达情况。方法:采用端粒重复序列扩增—聚合酶链反应—酶联免疫吸附测定(TRAP-PCR-ELISA)方法半定量检测CD4+T淋巴细胞和CD8+T淋巴细胞的端粒酶活性。结果:CD4+T淋巴细胞端粒酶阳性率43.8%(7/16),CD8+T淋巴细胞端粒酶阳性率37.5%(6/16)。结论:正常人外周血CD4+T淋巴细胞和CD8+T淋巴细胞可表达端粒酶活性。 Objective: To explore the characteristics of telomerase activity of human peripheral blood T lymphocytes. Methods: Telomerase activity of T lymphocytes was determined by polymerase chain reactionbased enzyme immunoassay (PCR-ELISA) using telomerase repeat amp lification protocol (TRAP). Results: The positive rates of telomerase activity of human peripheral blood CD4+T and CD8+T lymphocytes were 43.8% (7/16) and 37. 5% (6/16) .respectively. Conclusion: Human peripheral blood CD4+ T and CD8+T lymphocytes express telomerase activity.
出处 《广西医科大学学报》 CAS 2012年第5期681-683,共3页 Journal of Guangxi Medical University
基金 广西科学基金资助项目(No.桂科自06400960) 广西科学研究与技术开发计划项目(No.桂科自0007026)
关键词 端粒酶 CD4+T淋巴细胞 CD8+T淋巴细胞 telomerase CD4+ T lymphocytes CD8+ T lymphocytes
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参考文献10

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