摘要
从采自金华市各地的68份土样中分离得到了放线菌406株,以水稻纹枯病菌为指示菌,利用平皿对峙法筛选到42株具有较好拮抗作用的放线菌菌株.其中,Sh-43菌株的拮抗作用最强,抑菌带宽度为28.3mm.根据培养特征、形态特征、生理生化特征及16S rDNA序列比对分析,鉴定Sh-43菌株为吸水链霉菌(Strep-tomyces hygroscopicus).
Four hundred and six actinomycetes were isolated from sixty eight soil samples which were collected from different areas of Jinhua. Forty-two strains had been proved to have antagonistic activity against Rhizocto- nia solani by confronting cultivation on PDA plates. Among them, the strain Sh-43 exhibited the strongest an- tibiosis capacity with an inhibition zone of 28.3 mm. The strain Sh-43 was identified as scopicus on the cultural, morphological, physiological, biochemical characteristics and 16S rDNA gene se- quence analysis.
出处
《浙江师范大学学报（自然科学版）》
CAS
2012年第4期441-445,共5页
Journal of Zhejiang Normal University:Natural Sciences
基金
浙江省公益性应用研究计划项目(2010C32076)
关键词
纹枯病菌
放线菌
拮抗作用
筛选
鉴定
Rhizoctonia solani
actinomycete
antagonistic action
screening
identification
