摘要
目的探讨病理性疼痛和脾切除术对老年雄性大鼠海马细胞胆碱乙酰转移酶和细胞凋亡的不同影响及其机理。方法选择60只老年雄性大鼠,随机分为3组,A组单纯麻醉,只给予麻醉处理(单纯麻醉组);B组在左侧大腿中部切开,暴露坐骨神经后用4-0丝线结扎4道,常规缝合皮肤(CCI组);C组给予脾切除手术之后常规缝合伤口(脾切除组)。三组再按术前1d、术后1d、7d、14d4个时间点,将每组再分为4个小组(n=5)。分别为:A1、A2、A3、A4,B1、B2、B3、B4,C1、C2、C3、C4,在术前1d和术后1d、7d、14d按时间点经10%水合氯醛腹腔注射麻醉后,开胸经升主动脉依次灌注生理盐水100ml和4%多聚甲醛-PBS复合液300ml后,开颅取脑,石蜡切片后分别进行HE和CHAT免疫组化染色,光镜下观察海马组织细胞形态变化。结果 HE染色显示CCI组锥体层细胞损伤重,有较多凋亡形态细胞出现,高峰在术后14d,脾切除组锥体层和颗粒层均有损伤,出现细胞减少,形态改变;CHAT免疫组化染色3组均有CHAT阳性细胞数的减少,术后7d、14dCCI组较脾切除组染色明显偏浅。结论病理性疼痛和脾切除组造成海马细胞损伤的位置和程度不同,可能存在不同的机制。
Objective To investigate the different effects of pathological pain and splenectomy on the choline acetyltransferase and the apoptosis in hippocampus cells of aged male rats, and to investigate the mechanisms. Methods Sixty aged male rats were divided into three groups： group A, group B and group C. The rats in group A were Ireated with anesthesia simply, and those in group B were cut in the middle of the left thigh, and ligated 4 times with 4-0 silk thread af- ter the exposure of sciatic nerve, with the skin sutured routinely then. Rats in group C were given spleen surgery and rou- tine suture of the skin. The three groups were each divided into 4 sub-groups according to different time points （one day be- fore operation, 1 day, 7 days, 14 days after operation）, namely A 1, A2, A3, A4, B 1, B2, B3, B4, C 1, C2, C3, C4, with 5 rats in each sub-group. The rats in each group was anesthetized by 10% chloral hydrate at 4 time points, and then infused by 100 ml saline and 300 ml 4% POM-PBS composite though the ascending aorta. Craniotomied for brain, paraffin sections were carried out after HE and CHAT immunohistochemical staining. The changes of the hippocampus morphology were observed under a light microscope. Results In HE staining revealed that pyramidal layer injured badly in group B, which reached the peak 14 days after the operation, with a lot of apoptotic cells. In group C, pyramidal layer and granular layer were both injured badly, with the number of cells reduced and the cell morphology changed. The number of positive cells reduced in the three groups in CHAT immunohistochemical staining. The dye color of group B was lighter than that in group C 7 days and 14 days after operation. Conduslon The location and extent of the injury of the hippocampal ceils caused by pathological pain and splenectomy were different, with different mechanisms.
出处
《海南医学》
CAS
2012年第24期17-19,共3页
Hainan Medical Journal
