摘要
将李斯特菌噬菌体经口服灌入小鼠体内,分析噬菌体在小鼠体内的消长规律及特异性免疫应答,探索噬菌体作为生物抗菌剂在食品生物防控中的安全性。分别将噬菌体LipG2-5以1×108PFU单次及连续3次灌服小鼠,并在灌服后不同时间点检测粪样中噬菌体的数量并对小鼠进行称质量。对小鼠增质量分析表明,摄入噬菌体组小鼠体质量与对照组无显著性差异。在不同时间点采集小鼠血清及肠道黏膜,分析其特性抗体产生情况。结果表明,噬菌体灌服后随着时间的推移,其数量相应下降。在第7天,两组噬菌体摄入组粪样中均检测不到噬菌体。血清ELISA检测结果表明,灌服噬菌体小鼠能够产生较低水平的噬菌体特异性抗体,但与对照组无显著性差异。同样,在第35天并没有检测到较高水平的黏膜IgA抗体。由此可见,噬菌体在进入机体后,可以通过消化道自然排出,在体内无残留,不产生抗体。
To explore the safety of Listeria phage as a biological antimicrobic agent,its elimination and immune responses to it in BALB/c mice were analyzed.Mice were immunized by oral single-dose or triple-dose（once daily on 3 consecutive days） administration of 1×108 PFU of phage LipG2-5 and the number of phages in mouse feces sampled at different time points following administration was examined and body weight was measured.Phage treatment groups showed no significant difference in body weight from control group.Meanwhile,serum and intestinal mucosa were collected for analysis of the production of specific antibodies.As a result,the number of phages declined over time after administration and was no longer detectable in mouse feces from 7 until 35 d after administration.ELISA analysis indicated that oral administration of phages induced a low level of specific antibodies in the serum of mice without significant differences compared with control group.Similarly,the measured level of mucosal IgA 35 d after administration was not high.From these results,we conclude that Listeria phage can be directly excreted through the digestive tract without being retained in the body and inducing antibodies.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第21期169-172,共4页
Food Science
基金
国家自然科学基金青年基金项目(31101291)
江苏省农业科技自主创新项目(cx(11)4068)
