摘要
种子及胚胎发育是被子植物个体发育的重要起始阶段,相关调控基因的阐明将有助于认识种子及胚胎发育的分子机制。预测同源基因CHR12和CHR23编码拟南芥依赖于ATP的染色质重塑核心组分——腺苷三磷酸酶。T-DNA插入缺失突变体鉴定、遗传杂交及转基因实验表明,两个基因同时缺失阻滞种子的正常发育;胚胎发育进程显微观察表明,与野生型及各自单缺失突变体相比,双重缺失突变体的胚胎发育停滞到了心形胚后期或鱼雷胚早期。这表明CHR12和CHR23在拟南芥种子及胚胎发育过程中功能冗余地发挥着重要调控作用。
The research on the key regulating genes will help to detect the molecular mechanism in develop- ment of seed and embryo, the first stage of angiosperm ontogeny. Two highly homologous genes, CHR12 and CHR23, were predicted to code chromatin-remodeling core ATPase in Arabidopsis. The data, from T-DNA in- serted mutants, genetic cross and transgenic rescue experiments, indicated that chr12chr23, double loss-of- function mutants of CHRI2 and CHR23, resulted in seed defect. Further microscopical observation about the process of embryo development showed that the embryo of chr12chr23 only develop into heart-stage and early torpedo-stage. All these data suggest CHR12 and CHR23 play redundant roles in the developmental process of Arabidopsis seed and embryo.
出处
《植物生理学报》
CAS
CSCD
北大核心
2012年第11期1084-1090,共7页
Plant Physiology Journal
基金
国家自然科学基金项目(31171170)
河北省科技厅重点基础研究项目(10960121D)
