摘要
目的探讨前列腺癌中XAF1 mRNA表达及DNA甲基化抑制剂对其表达的影响。方法培养前列腺癌细胞株LNCaP、DU145和PC3及肾癌细胞株A498,提取正常人外周血单核细胞(PBMC)、A498和PBMC用作阳性对照。搜集临床10例前列腺癌及邻近前列腺组织作对照。RT-PCR法检测各细胞株和正常人外周血单核细胞中XAF1 mRNA表达,甲基化特异性PCR法检测甲基化酶抑制剂(5'-aza)处理前后前列腺癌细胞株LNCaP、DU145中XAF1 mRNA表达。结果 LNCaP和DU145中XAF1 mRNA的表达水平低于肾癌细胞株A498,PC3中XAF1mRNA不表达。8例患者中有6例XAF1 mRNA在癌组织中的表达低于邻近前列腺组织。使用5'-aza处理后的LNCaP和DU145中XAF1 mRNA均以全长形式表达。结论 XAF1 mRNA在LNCaP和DU14中均为低表达,在PC3不表达。DNA 5'-aza可诱导前列腺癌细胞株中XAF1 mRNA的全长表达。
Objective To investigate the expression of XAF1 mRNA and the effect of DNA methylation inhibitor on its expression in prostate cancer. Methods Culture prostate cancer cell lines LNCaP, DU145, PC3 cells and renal cell carcinoma-derived A498 cells. Extract normal peripheral blood mononuclear cells （PBMCs）. A498 cells and PBMCs were used as positive controls. Collect 10 clinical cases of prostate cancer and neighboring prostate tissue as control. RT-PCR was performed in order to detect the XAF1 mRNA experssion in LNCaP, DU145, PC3, A498 and PBMCs. Methylation-specific PCR（MSP） was used to detect the XAF1 experssion in LNCaP and DU145 cells before and after they were treated with DNA methylation inhibitor （5＇-aza）. Results LNCaP and DU145 prostate cancer cell lines ex- pressed a shorter form of XAF1 transcripts than the A498 cells, whereas PC3 cells exhibited a complete silence of the XAF1 gene. Yet,after being treated with 5＇-aza, LNCaP and DU145 expressed the full length of XAF1 mRNA. Con- clusion Low expression of XAF1 mRNA is found in LNCaP and DU14 cells. No expression is found in PC3 cells. DNA methylation inhibitor can induce the full-length of XAF1 mRNA expression in prostate cancer cells.
出处
《山东大学学报（医学版）》
CAS
北大核心
2012年第12期94-97,102,共5页
Journal of Shandong University:Health Sciences
基金
国家自然科学基金(81172435)
