摘要
目的:研究PHLPP1在人脑胶质瘤组织中的表达及其临床意义。方法:选取徐州医学院附属医院神经外科2009年12月至2011年10月间手术切除并经病理证实的人脑胶质瘤标本37例,其中I级1例,Ⅱ级12例,Ⅲ级16例,胶质母细胞瘤8例。另取11例因脑创伤行内减压术患者的正常脑组织标本作为对照。应用RT-PCR、Western blotting分别检测各标本中PHLPP1 mRNA和PHLPP1蛋白的表达。结果:RT-PCR和Western-blotting检测结果显示正常脑组织标本中PHLPP1 mRNA和PHLPP1蛋白表达最高,不同级别胶质瘤组织PHLPP1 mRNA和PHLPP1蛋白表达差异有统计学意义(P<0.05),且随着肿瘤病理级别的增高,胶质瘤组织中PHLPP1 mRNA和PHLPP1蛋白表达值降低,表达差异有统计学意义(P<0.05)。结论:PHLPP1在正常脑组织和不同级别的脑胶质瘤组织中都有表达,其表达值与肿瘤的病理分级呈负相关性,这可能是胶质瘤发生发展的重要机制之一。
Objective：To investigate the expression of PHLPP1 in the tumor tissues of patients with gliomas and evaluate its clinical significance.Methods： Thirty-seven patients with brain gliomas（1 with grade I,12 grade Ⅱ,16 grade Ⅲ and 8 with glioblastoma） were performed surgical excision in our hospitals from December 2009 to October 2011 were chosen and 11case of normal brain tissues from patients performed decompression operation resulting from cerebral hernia were selected as the controls.RT-PCR and Western-blotting were used to detect mRNA and protein expressions of PHLPP1.Results： The results investigated by RT-PCR and Westem-blotting were consistent,the highest expression of PHLPP1 was in the normal brain tissues.The expression value between different grades of tumors was obviously significant and decreased in a grade-dependent manner（P0.05）.Conclusion： The mRNA and protein expressions of PHLPP1 are noted with positive correlation to the pathological grades of gliomas,indicating that PHLPP1 may play an important role in the generation and development of gliomas.
出处
《现代肿瘤医学》
CAS
2012年第12期2495-2497,共3页
Journal of Modern Oncology
基金
江苏省研究生科技创新基金(编号:CXLX11-0738)
