摘要
以鹿角杜鹃新生嫩叶为外植体,通过均匀设计法筛选其最适合的愈伤组织诱导、愈伤组织再分化及生根培养基。结果表明,最适合鹿角杜鹃嫩叶愈伤组织诱导的培养基为:DR+ZT2.60 mg·L-1+IAA0.75 mg·L-1,诱导率为99.2%;愈伤组织再分化培养基为:DR+ZT3.35 mg·L-1+IAA 0.05 mg·L-1+KT 1.90 mg·L-1,分化率为99.9%;生根培养基为:1/4DR+KT0.10 mg·L-1+IBA0.04 mg·L-1,生根率达98.0%以上。以再生植株茎节为材料进行快繁的结果表明,在38 d的培养周期内,每段增殖倍数平均达7以上,再生率为96.5%。再生植株的炼苗栽培成活率达98.5%以上,并成功建立了鹿角杜鹃嫩叶的愈伤组织诱导、再分化芽苗和高效植株再生体系,所建立的高效植株再生体系可满足于鹿角杜鹃工厂化育苗的需要。
New leaves of Rhododendron latoucheae were used as explants for the experiment, and culture media for callus induction, callus redifferentiation and rooting were screened through uniform design. Results showed that DR supplemented with 2.60 mg . L-1 ZT and 0.75 mg . L-1 IAA was the best medium for callus induction from new leaves, with a callus induc- tion frequency of 99.2%. DR containing 3.35 mg . L-1 ZT, 0.05 mg . L-1 IAA and 1.90 mg . L-l KT was the optimal medium for shoot redifferentiation, with a callus differentiation rate of 99.9%. 1/4DR supplemented with 0.10 mg . L-1 KT and 0.04 mg~ L-l IBA was the best medium for rooting, with a rooting rate of more than 98.0%. Stems each with one node were cut from regenerated shoots and cultured for propagation, and a 7-fold proliferation rate was achieved within 38 days, and the rate of plantlet regeneration was 96.5%. The survival rate of regenerated plants was more than 98.5%. In this study, callus induction, shoot redifferentiation from the new leaves of R. latoucheae and efficient regeneration system of plantlet were successfully established, which could meet the requirements for raising seedlings of R. latoucheae in industrial scale.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2012年第12期51-55,共5页
Journal of Northeast Forestry University
基金
吉林省科技发展计划项目(200705C05)
关键词
鹿角杜鹃
离体培养
愈伤组织
植株再生
均匀设计
Rhododendron latoucheae
in vitro culture
Callus
Planflet regeneration
Uniform design
