摘要
为提高草鱼下脚料鱼鳔中胶原蛋白的提取率,采用响应面法优化胃蛋白酶提取草鱼鱼鳔胶原蛋白。探讨加酶量、提取时间和液料比3个因素对提取率的影响;在单因素试验的基础上进行响应面优化试验。结果表明:胃蛋白酶提取草鱼鱼鳔胶原蛋白的最佳工艺条件为加酶量40mg/g、提取时间48h、液料比80:1(mL/g),在此工艺条件下胶原蛋白实际提取率为17.82%,与模型预测值(18.04%)接近。方差分析显示,模型可靠,3个因素均对草鱼鱼鳔胶原蛋白的提取率影响显著。
The pepsin-catalyzed hydrolysis of grass carp bladders was optimized by response surface methodology to improve the extraction yield of collagen.We explored the effects of hydrolysis parameters,enzyme loading,hydrolysis time and liquid-to-solid ratio on the extraction yield of collagen.Their optimal values for collagen extraction were found to be 40 mg/g,48 h and 80：1（mL/g）,respectively.Under the optimized conditions,the actual extraction yield of collagen was 17.82%,which was close to the predicted value（18.04%）.The results of analysis of variance showed that the fitted regression model was reliable,and each hydrolysis parameters had a significant effect on the extraction yield of collagen.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第22期154-157,共4页
Food Science
关键词
响应面优化
胃蛋白酶
草鱼鱼鳔
胶原蛋白
response surface methodology
pepsin
grass carp bladders
collagen
