摘要
从加油站附近的被污染土壤中分离到9株柴油降解菌,选取降解率较好的菌A、菌C,根据其形态观察、生理生化特性以及16S rDNA基因序列相似性分析,初步鉴定为芽孢杆菌属,菌A为短小芽孢杆菌,菌C为枯草芽孢杆菌。单因素实验表明,在10 mL/L的柴油降解培养基中,菌A的最适温度为37℃,pH为8.0,接种量15%,第7 d降解率达到42%;菌C的最适温度为37℃,pH为7.5,接种量10%,第7 d降解率达到40%。这两株菌在柴油降解过程中都会产生脂肽类表面活性剂,引起表面张力下降,柴油降解培养基最初的表面张力是64.13 mN/m,5 d后,菌A和菌C表面张力分别下降到37.61 mN/m,42.02 mN/m。
Nine bacteria strains were isolated from the contaminated soil near the fueling station. Strain A and C with relatively higher diesel oil biodegradation ability were chosen for further study. They were identified as Bacillus sp. based on their morphological, physiological, biochemical features and 16S rDNA sequences. Strain A was B. pumilus and strain C was B. subtilis. Parameters affecting biodegradation activity were investigated to obtain the optimal degradation condition. Under the optimal degradation conditions, the degradation rates of strain A and C after 7 clays were 42% and 40%, respectively. The results showed that both strain A degradation, resulting in the surface tension decreased and C produce biosurfactant during the process of diesel oil from 64. 13 mN/m to 37. 61 mN/m and 42. 02 mN/m, respectively. FTIR results indicated that the biosurfactants prouduced were both lipopepitides.
出处
《工业微生物》
CAS
CSCD
2012年第6期14-20,共7页
Industrial Microbiology
基金
教育部新世纪优秀人才支撑计划
NcET-10-0435
关键词
柴油
生物降解
表面活性剂
diesel oil
biodegradation
biosurfactant
