摘要
采用高效液相色谱法 ,以HypersilC1 8( 4 6mmi d × 2 50mm ,5μm)为色谱柱 ,甲醇 水 磷酸 (体积比为 60∶40∶0 35,pH 3～ 4)为流动相 ,检测波长为 2 72nm ,测定了α 萘乙酸的质量分数。在 1 6mg/L～1 0 0 0mg/L时 ,质量浓度与峰面积线性关系良好 (r =0 9997) ,精密度RSD为 0 8% (n =5)。方法简便 ,准确。
A high performance liquid chromatographic method for the determination of α naphthylacetic acid has been established on a Hypersil C\-\{18\} column(4.6 mm i.d.×250?mm, 5?μm),with the mobile phase of methanol water phosphoric acid(60∶40∶0 35, V/V), and UV detection at 272?nm. The linear correlation was observed within the range of 16 mg/L 1 000?mg/L(r=0 999 7).The RSD was 0 8%(n=5).The method is simple and accurate.
出处
《色谱》
CAS
CSCD
北大核心
2000年第4期372-373,共2页
Chinese Journal of Chromatography
关键词
高效液相色谱法
Α-萘乙酸
测定
植物生长激素
high performance liquid chromatography
α naphthylacetic acid
β naphthylacetic acid
