摘要
考察了用微乳液毛细管电动色谱 ( MEEKC)分离蛋白质时微乳液组成等不同因素对分离的影响 ,并与胶束电动色谱进行对比 ,探讨了其分离机理 。
Microemulsion electrokinetic chromatography(MEEKC) is a new technique which was employed to separate both basic and acidic proteins simultaneously. Optimum separation conditions were investigated with respect to ionic strength and the composition of microemulsion. The results show that if a stable microemulsion formed, the efficiency of protein separation was slightly affected by the concentration of the background and the composition of microemulsion. The fundamental characteristics of MEEKC were studied in comparison with micellar electrokinetic chromatography(MEKC). The mechanism of MEEKC was also investigated. The results show that MEEKC is an alternative way for protein separations with a high efficiency.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2000年第8期1183-1186,共4页
Chemical Journal of Chinese Universities
基金
国家自然科学基金! (批准号 :692 3 5 0 2 2 0 )
关键词
微乳液毛细管电动色谱
蛋白质
分离
MEKC
Microemulsion electrokinetic chromatography
Micellar electrokinetic chromatography
Protein
