摘要
目的:建立孕妇清火丸中芒果苷的含量测定方法。方法:采用高效液相色谱法,Thermo BDS HYPERSIL C18色谱柱,乙腈-0.2%冰醋酸溶液(15∶85)为流动相,流速1.0 mL.min-1,检测波长258 nm。结果:芒果苷在0.053 50～0.374 5μg线性关系良好(r=0.999 8),平均回收率为99.22%,RSD 1.15%(n=6)。结论:该方法简便、准确,重复性好,可用于孕妇清火丸的质量控制。
Objective： To establish a method for the determination of mangiferin in Yunfu Qinghuo pills. Method： Using HPLC, chromatographic conditions： the chromatographic system consisted of Thermo BDS HYPERSIL Clscolumn, using acetonitrile-0.2% acetic acid glacial （15： 85） as mobile phase. The content was detected at a wavelength of 258 nm, the flow rate was 1.0 mL·min^-1 Result： Mangiferin was linear in the range of 0.053 50-0. 374 5 Ixg （r =0.999 8）, the average recovery was 99.22% and RSD was 1.15% （n =6）. Conolusion ： The method is simple, accurate, reproducible and can be used for the determination of the content of mangiferin in Yunfu Qinghuo Pills.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第24期146-148,共3页
Chinese Journal of Experimental Traditional Medical Formulae
