摘要
目的建立鼠曲草中槲皮素的含量测定方法。方法采用VP-ODS C18(4.6 mm×150 mm,5μm)色谱柱。以甲醇-0.2%磷酸溶液(45∶55)为流动相;流速为1.0 ml/min;检测波长为375 nm。结果鼠曲草中的槲皮素含量测定方法在10.22～51.10μg/ml浓度范围内与峰面积的线性关系良好(r=0.999 7);平均回收率为100.18%,RSD为1.38%。结论所建立的方法准确、灵敏、重现性好,可用于鼠曲草的质量控制。
Objective To establish the determination method of Quercetin content in Gnaphalium affine D. Don. Methods VP-ODS CL8 （4.6 mm× 150mm,5 μm） colmnn was used, the mobile phase was methanol-0.2% phosphoric acid solution（45 ： 55） with the flow rate as 1.0 ml/min. The detection wavelength was 375 ran. Results The linear relation of byperin was good in the range from 10.22 μg/ml to 51.10 μg/ml. The average recovery was 100. 18% , RSD was 1.38%. Conclusion The method established was accurate, sensitive and good in reproducibility which could be applied to the quality control of G. affine D. Don.
出处
《药学实践杂志》
CAS
2012年第6期451-453,共3页
Journal of Pharmaceutical Practice
